Literature DB >> 3593682

Isolation of full-length putative rat lysophospholipase cDNA using improved methods for mRNA isolation and cDNA cloning.

J H Han, C Stratowa, W J Rutter.   

Abstract

We have cloned a full-length putative rat pancreatic lysophospholipase cDNA by an improved mRNA isolation method and cDNA cloning strategy. These new methods allow the construction of a cDNA library from the adult rat pancreas in which the majority of recombinant clones contained complete sequences for the corresponding mRNAs. A previously recognized but unidentified long and relatively rare cDNA clone containing the entire sequence from the cap site at the 5' end to the poly(A) tail at the 3' end of the mRNA was isolated by single-step screening of the library. The size, amino acid composition, and the activity of the protein expressed in heterologous cells strongly suggest this mRNA codes for lysophospholipase [Van den Bosch, H., Aarsman, A. J., DeJong, G. N., & Van Deenen, L. M. (1973) Biochim. Biophys. Acta 296, 94-104].

Entities:  

Mesh：

Substances：

Year:  1987        PMID： 3593682     DOI： 10.1021/bi00380a020

Source DB:  PubMed          Journal:  Biochemistry        ISSN： 0006-2960            Impact factor:   3.162


  78 in total

1.  Molecular cloning of chicken aggrecan. Structural analyses.

Authors:  L Chandrasekaran; M L Tanzer
Journal:  Biochem J       Date:  1992-12-15       Impact factor: 3.857

2.  Brain and gastrointestinal cholecystokinin receptor family: structure and functional expression.

Authors:  S A Wank; J R Pisegna; A de Weerth
Journal:  Proc Natl Acad Sci U S A       Date:  1992-09-15       Impact factor: 11.205

3.  Cloning and cDNA sequence of a bovine submaxillary gland mucin-like protein containing two distinct domains.

Authors:  A K Bhargava; J T Woitach; E A Davidson; V P Bhavanandan
Journal:  Proc Natl Acad Sci U S A       Date:  1990-09       Impact factor: 11.205

4.  Cloning and sequencing of rat plectin indicates a 466-kD polypeptide chain with a three-domain structure based on a central alpha-helical coiled coil.

Authors:  G Wiche; B Becker; K Luber; G Weitzer; M J Castañon; R Hauptmann; C Stratowa; M Stewart
Journal:  J Cell Biol       Date:  1991-07       Impact factor: 10.539

5.  Reverse transcriptase. The use of cloned Moloney murine leukemia virus reverse transcriptase to synthesize DNA from RNA.

Authors:  G F Gerard; D K Fox; M Nathan; J M D'Alessio
Journal:  Mol Biotechnol       Date:  1997-08       Impact factor: 2.695

6.  Pancreatic acinar cells produce, release, and respond to tumor necrosis factor-alpha. Role in regulating cell death and pancreatitis.

Authors:  A S Gukovskaya; I Gukovsky; V Zaninovic; M Song; D Sandoval; S Gukovsky; S J Pandol
Journal:  J Clin Invest       Date:  1997-10-01       Impact factor: 14.808

7.  Cholinesterase-like domains in enzymes and structural proteins: functional and evolutionary relationships and identification of a catalytically essential aspartic acid.

Authors:  E Krejci; N Duval; A Chatonnet; P Vincens; J Massoulié
Journal:  Proc Natl Acad Sci U S A       Date:  1991-08-01       Impact factor: 11.205

8.  Human organ-specific autoimmune disease. Molecular cloning and expression of an autoantibody gene repertoire for a major autoantigen reveals an antigenic immunodominant region and restricted immunoglobulin gene usage in the target organ.

Authors:  G D Chazenbalk; S Portolano; D Russo; J S Hutchison; B Rapoport; S McLachlan
Journal:  J Clin Invest       Date:  1993-07       Impact factor: 14.808

9.  Presence in human eosinophils of a lysophospholipase similar to that found in the pancreas.

Authors:  F W Holtsberg; L E Ozgur; D E Garsetti; J Myers; R W Egan; M A Clark
Journal:  Biochem J       Date:  1995-07-01       Impact factor: 3.857

10.  Molecular cloning, tissue distribution, and expression of a 14-kDa bile acid-binding protein from rat ileal cytosol.

Authors:  Y Z Gong; E T Everett; D A Schwartz; J S Norris; F A Wilson
Journal:  Proc Natl Acad Sci U S A       Date:  1994-05-24       Impact factor: 11.205
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.