Full utilization of marine microalgal hydrothermal liquefaction liquid products through a closed-loop route: towards enhanced bio-oil production and zero-waste approach.

The present study aimed to evaluate the potential of aqueous phase after hydrothermal liquefaction of microalgae (Aq-P), enriched with seawater, as a growth medium coupled with crude bio-oil production by the halophyte Dunaliella salina. Results showed that Aq-P showed higher content of total organic carbon (TOC) and total nitrogen (10.24, and 5.11 g L-1, respectively), while seawater showed higher anions and cations content. At the 12th day of microalgae incubation, the Aq-P growth medium showed 15.9% higher dry weight than the control (f/2 medium), with enhanced lipid content by 21.2% over the control, and 5.7% significant reduction in carbohydrates. The bio-oil yields of microalgal biomass cultivated in f/2 and Aq-P were 28.74% and 29.54%, respectively. Using Aq-P enhanced the fatty acids/esters and hydrocarbons in the crude bio-oil by 12.6% and 1.7 times, respectively, comparing to f/2-derived bio-oil. However, nitrogen-containing compounds in the Aq-P-derived bio-oil reduced by 60.7% comparing to f/2 medium. Interestingly, diesel carbon-range represented the majority of the products in both f/2- and Aq-P-derived bio-oil (69.1% and 78.3%, respectively). The findings of the present study provide a new approach for development of sustainable microalgal cultivation system for crude bio-oil production through a closed-loop route using Aq-P and seawater. © King Abdulaziz City for Science and Technology 2022, Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.