Literature DB >> 3592910

Characterization of two ornithine carbamoyltransferases from Pseudomonas syringae pv. phaseolicola, the producer of phaseolotoxin.

O Jahn, J Sauerstein, G Reuter.   

Abstract

Two ornithine carbamoyltransferases (OCT 1 and OCT 2) were isolated from Pseudomonas syringae pv. phaseolicola and purified by precipitation with ammonium sulfate, heat denaturation, chromatography on DEAE-Sephadex A-50 and Sephadex G-200. Molecular weights of both enzymes: 110,000; optimal activity: pH 8.5 to 9.5 (OCT 1), pH 8.4 (OCT 2); apparent Km for ornithine: 7 X 10(-4) (both enzymes); apparent Km for carbamoyl-phosphate: 7 X 10(-4) (OCT 1), 2.8 X 10(-3) (OCT 2). Both enzymes possess only an anabolic function. OCT 1 is highly inhibited by low concentrations of phaseolotoxin and Orn-P(O)(NH2)-NH-SO3H, OCT 2 is insensitive to both compounds. The inhibition of OCT 1 is reversible.

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Year:  1987        PMID： 3592910     DOI： 10.1007/bf00415280

Source DB:  PubMed          Journal:  Arch Microbiol        ISSN： 0302-8933            Impact factor:   2.552


  10 in total

1.  Mode of Action of the Toxin from Pseudomonas phaseolicola: II. Mechanism of Inhibition of Bean Ornithine Carbamoyltransferase.

Authors:  L Q Tam; S S Patil
Journal:  Plant Physiol       Date:  1972-05       Impact factor: 8.340

2.  Inhibition of Ornithin Carbamyl Transferase from Bean Plants by the Toxin of Pseudomonas phaseolicola.

Authors:  S S Patil
Journal:  Plant Physiol       Date:  1970-11       Impact factor: 8.340

3.  Structure and function of ornithine carbamoyltransferases.

Authors:  C Legrain; V Stalon; J P Noullez; A Mercenier; J P Simon; K Broman; J M Wiame
Journal:  Eur J Biochem       Date:  1977-11-01

4.  The specialization of the two ornithine carbamoyltransferases of Pseudomonas.

Authors:  F Ramos; V Stalon; A Piérard; J M Wiame
Journal:  Biochim Biophys Acta       Date:  1967-05-16

5.  Taxonomy of phytopathogenic pseudomonads.

Authors:  D C Sands; M N Schroth; D C Hildebrand
Journal:  J Bacteriol       Date:  1970-01       Impact factor: 3.490

6.  Detection of two ornithine carbamoyltransferases in a phaseolotoxin-producing strain Pseudomonas syringae pv. phaseolicola.

Authors:  O Jahn; J Sauerstein; G Reuter
Journal:  J Basic Microbiol       Date:  1985       Impact factor: 2.281

7.  Phaseolotoxin-insensitive ornithine carbamoyltransferase of Pseudomonas syringae pv. phaseolicola: basis for immunity to phaseolotoxin.

Authors:  B J Staskawicz; N J Panopoulos; N J Hoogenraad
Journal:  J Bacteriol       Date:  1980-05       Impact factor: 3.490

8.  Inactivation of bean ornithine carbamoyltransferase by phaseotoxin: effect of phosphate.

Authors:  O C Kwok; H Ako; S S Patil
Journal:  Biochem Biophys Res Commun       Date:  1979-08-28       Impact factor: 3.575

9.  Involvement of phaseolotoxin in halo blight of beans: transport and conversion to functional toxin.

Authors:  R E Mitchell; R L Bieleski
Journal:  Plant Physiol       Date:  1977-11       Impact factor: 8.340

10.  Mode of Action of the Toxin from Pseudomonas phaseolicola: I. Toxin Specificity, Chlorosis, and Ornithine Accumulation.

Authors:  S S Patil; L Q Tam
Journal:  Plant Physiol       Date:  1972-05       Impact factor: 8.340
  10 in total
  7 in total

1.  The global arginine regulator ArgR controls expression of argF in Pseudomonas syringae pv. phaseolicola but is not required for the synthesis of phaseolotoxin or for the regulated expression of argK.

Authors:  José Luis Hernández-Flores; Karina López-López; Rogelio Garcidueñas-Piña; Alba E Jofre-Garfias; Ariel Alvarez-Morales
Journal:  J Bacteriol       Date:  2004-06       Impact factor: 3.490

2.  Origin, structure, and regulation of argK, encoding the phaseolotoxin-resistant ornithine carbamoyltransferase in Pseudomonas syringae pv. phaseolicola, and functional expression of argK in transgenic tobacco.

Authors:  E Hatziloukas; N J Panopoulos
Journal:  J Bacteriol       Date:  1992-09       Impact factor: 3.490

3.  Partial characterization of ornithine carbamoyltransferase in three microalgae : anabolic role only.

Authors:  G Laliberté; J A Hellebust
Journal:  Plant Physiol       Date:  1990-05       Impact factor: 8.340

4.  Functional characterization of the gene cluster from Pseudomonas syringae pv. phaseolicola NPS3121 involved in synthesis of phaseolotoxin.

Authors:  Selene Aguilera; Karina López-López; Yudith Nieto; Rogelio Garcidueñas-Piña; Gustavo Hernández-Guzmán; José Luis Hernández-Flores; Jesús Murillo; Ariel Alvarez-Morales
Journal:  J Bacteriol       Date:  2007-01-19       Impact factor: 3.490

5.  In Pseudomonas syringae pv. phaseolicola, expression of the argK gene, encoding the phaseolotoxin-resistant ornithine carbamoyltransferase, is regulated indirectly by temperature and directly by a precursor resembling carbamoylphosphate.

Authors:  Karina López-López; José Luis Hernández-Flores; Marisa Cruz-Aguilar; Ariel Alvarez-Morales
Journal:  J Bacteriol       Date:  2004-01       Impact factor: 3.490

6.  Expression of the gene for resistance to phaseolotoxin (argK) depends on the activity of genes phtABC in Pseudomonas syringae pv. phaseolicola.

Authors:  Selene Aguilera; Susana De la Torre-Zavala; José Luis Hernández-Flores; Jesús Murillo; Jaime Bravo; Ariel Alvarez-Morales
Journal:  PLoS One       Date:  2012-10-08       Impact factor: 3.240

7.  Temperature-mediated biosynthesis of the phytotoxin phaseolotoxin by Pseudomonas syringae pv. phaseolicola depends on the autoregulated expression of the phtABC genes.

Authors:  Selene Aguilera; Ariel Alvarez-Morales; Jesús Murillo; José Luis Hernández-Flores; Jaime Bravo; Susana De la Torre-Zavala
Journal:  PLoS One       Date:  2017-06-01       Impact factor: 3.240
  7 in total

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