Diagnostic utility of conventional and liquid-based cytology in the management of thyroid lesions; an institutional experience.

Objectives: Liquid-based cytology (LBC) is becoming a useful method in evaluating both gynecological and nongynecological preparations. Many studies have been conducted these days to see the quality of LBC in diagnosing thyroid lesions and its role is yet to be standardized. This study has been done to pick the cytomorphological features of thyroid lesions on LBC and to compare them with the conventional cytology of these lesions.The objective of the study is to evaluate the efficacy of thyroid fine-needle aspiration cytology processed by LBC as compared to conventional smears. Material and
Methods: A prospective study has been done on 53 cases of thyroid lesions using standard conventional and LBC techniques.
Results: In most of the cases, the cytological features are similar in conventional cytology smears and LBC, but the colloid film and the lymphocytic component show a clear picture on direct smears, whereas nuclear and cytoplasmic details are better evaluated in LBC slides. The preservative solution used in LBC could be efficiently used for the application of immunocytochemical and molecular techniques.
Conclusion: LBC does not represent as a valid alternative to conventional cytology but it can be considered as a diagnostic utility process especially due to the possibility of applying additional techniques which enhance the efficacy of the cytological diagnosis of thyroid lesions.

INTRODUCTION

Fine-needle aspiration cytology (FNAC) is a fast, simple, and cost-effective preliminary procedure where cells are obtained from any lesion using a thin bore needle, smears are prepared and stained to see under the microscope to reach out a diagnosis. This technique carries lesser complications and can be performed in day care. This technique is based on the fact that tumor cells are less cohesive and are easily aspirated. This is used in the diagnosis of breast lumps, thyroid nodules, liver disease, subcutaneous soft tissue mass, salivary gland diseases, and oral diseases.

Martin and Ellis introduced this technique first in the 1930s in the United States, but it never became widespread. Since the 1950s it has been used extensively in Scandinavia and in Holland. Fine needle for aspiration was first introduced in Europe in the 1950’s by Lopez-Cardozo in the Netherlands and Soderstrom in Sweden. Zajicek’s publications from Karolinska Hospital in Stockholm that brought FNAC to international alterations. Since then, it has been extensively used as conventional cytological process to diagnose various pathologies.

The use of liquid-based cytology (LBC) is also gaining interest in the preoperative evaluation of thyroid lesions.[ The prevalence of thyroid nodules that are palpable is 12.2% worldwide and this number gets increased to 10–14% if detected by any imaging technique.[ The percentage of malignant nodules among thyroid lesions is around 2–7% only, mostly the thyroid nodules are benign.[ With the use of FNA, the resection of malignant thyroid nodules has been increased from 14% to 50%.[ Conventional cytological preparations overall have a lot of drawbacks such as dirty and hemorrhagic background which may make the diagnosis difficult. Adequacy is also of concern particularly so when the lesion is hemorrhagic, thus LBC came into existence.[ However, LBC has its own drawbacks and it often removes material which may be of diagnostic significance. It has also been reported that LBC probably changes the morphologic details making it tough to give a diagnosis without adequate exposure to LBC.[

Thus, we undertook a pilot study to evaluate the advantages and disadvantages of both the methods of cytology in Indian scenario. This study gives us an idea about the cytomorphological features of various thyroid lesions on conventional cytology and LBC, the comparison between the two methods, and the diagnostic utility of LBC for various ancillary techniques.

MATERIAL AND METHODS

A prospective study had been conducted in the Department of Pathology of Vardhman Mahavir Medical College & Safdarjung Hospital, New Delhi in 2015-2016 on 53 patients where the thyroid swelling was easily visible with the naked eye. In each one, after performing clinical examination like deglutition test, FNA was performed using a 23-gauge needle, 20-mL syringe, and syringe pistol without any USG guidance. In the first step, air-dried smears were made and stained with Giemsa, and alcohol-fixed smears were stained by Papanicolaou stain. Then, whatever material is left in the needle and syringe was transferred into 30 mL of CytoLyt solution (Cytyc) by rinsing the needle and syringe. It was centrifuged for 10 min at 500 g. The supernatant fluid was discarded and the material resuspended in a cytopreservative solution (PreservCyt, Cytyc). After 15 min, the material was processed in BD surepath SOP by standard operative procedure as described by the company. The remaining material was used to make additional smears for immunostaining. Following monoclonal antibodies were used on LBC and conventional cytology as and when indicated;

TTF 1

Thyroglobulin

LCA

Calcitonin

Kapa, Lambda (κ, λ)

T cell markers

B cell markers.

Immunocytochemistry (ICC) method was same as for conventional cytology. Two observers examined the conventional smears and LBCs on different occasions, without knowing the diagnosis. The representative CSs and LBCs were compared for adequacy, cellularity, background blood and cell debris, cell architecture, informative background (such as colloid), presence of cells in monolayer, and nuclear/cytoplasmic details.

Inclusion criteria

All thyroid lesions irrespective of types.

Exclusion criteria

Midline neck swelling that is not arising from thyroid (radiologically proven).

RESULTS

A total of 53 cases were diagnosed by LBC and conventional cytology [Table 1]. The largest number of cases were of colloid nodular goiter amounting to 50.9%. Hashimoto’s/ Lymphocytic thyroiditis which were 26.4% (n = 14). Neoplasms and thyroid hyperplasia were three cases each, i.e. 5.6%. A significant number of cases where no definitive diagnosis could be given were included under descriptive cytology. These were 11.2%.

Table 1:

Distribution of cases and their LBC findings.

S. No.	Cytological diagnosis	No. of cases	Percentage	Cytomorphological findings in Liquid Based Cytology
1.	Colloid nodular goitre	27	50.9	Colloid globules (small clumps of dense colloid)
2.	Hashimoto’s thyroiditis/lymphocytic thyroiditis	14	26.4	Lymphoepithelial clusters
3.	Neoplasms	3	5.7	Nuclear inclusions in Papillary carcinoma
4.	Hyperplasia	3	5.7	Fire flares
5.	Descriptive	6	11.3	Better adequacy

Conventional cytology preparation showing thyroid follicular cells and lymphocytic impingement and blood in the background.

Liquid-based cytology preparation showing thyroid follicular cells and lymphocytes with clear background.

FNA smears show thick colloid against the background of blood.

LBC smear shows colloid more clearly than the conventional FNA smears.

Liquid-based cytology preparation showing clearer background and better nuclear morphology of thyroid follicular cells as compared to conventional FNA smears.

Liquid-based cytology preparations positive for Immunocytochemistry chromogranin in a case of medullary carcinoma thyroid.

DISCUSSION

LBC has gained great success in recent years in gynecological cases. LBC is not only making the diagnosis easier for the pathologist but improving the turnaround time too because of single slide to screen. However, the cellularity in LBC and conventional cytology smears are similar but the nuclear details are clear in LBC.[ Almost no red blood cells in the background in comparison to the conventional smears where hemorrhagic background hampers the final impression in all the cases.[ In our study, a significant number of our cases (11.2%) were inadequate. However, a repeat slide preparation from the residual material made some of the cases adequate and a proper diagnosis could be made. The easiest lesion to diagnose was the colloid nodular goiter. However, compared to conventional cytology, in LBC colloid appears as small droplets. The morphology of thyroiditis cases was similar in both the methods, though the concentration of lymphocytes appears little more and lymphoepithelial clusters could be identified in LBC.

After making a cytological diagnosis, whatever material remaining in the vial can be used for the application of ancillary techniques such as ICC, flow cytometry, and molecular biology because the LBC method enables the storage of a variable number of cells for up to 6 months after the FNAC.[ We have used thyroid markers such as TTF 1, thyroglobulin, LCA, calcitonin which also appeared crisp and clear on LBC. Their visualization being better, helped us in clinching the diagnosis. The number of neoplastic lesions was less in our study, thus a significant comment on morphologic details is not possible. However, it was felt that nuclear groove and inclusions were better appreciated in LBC.[ Cell Block preparations, a powerful technique, is being used for ages for evaluating tissue morphology and performing immunostaining on sections.[ Cellblock immunohistochemistry is much more reliable owing to the gold standard of histological staining compared to conventional cytology and LBC.[ In a study conducted by Biscotti et al., cell types and cellular arrangements were well preserved in LBC slides. The study also stated that LBC and conventional smears have same diagnostic accuracy.[ No nuclear and cytoplasmic differences in LBC and conventional smears were noted by another study conducted by Mesonero and Sickel. The diagnostic correlation of both the methods was 90% as per their study.[ Frost and Cochand-Priollet et al. found variation in nuclear and cytoplasmic features among LBC and CS. Maximum number of cases in their study were turned out to be unsatisfactory.[

Conventional smears stained with Romanowsky stains or Papanicolaou stain are very well established to diagnose different categories of Bethesda system reporting. A lot of training is required to run both the methods in parallel which may help a reporting cytopathologists in gaining self-confidence and to make them familiar with the distinguishing cytomorphological features of the two methods.

CONCLUSION

LBC is not an alternative to conventional cytology but a good addition to it as it provides excellent nuclear, as well as cytoplasmic details and the drawbacks of conventional cytology such as drying artefact and hemorrhagic background are minimized. It also has an additional advantage of reutilizing the residual material thus preventing a repeat aspiration.

Table 2:

Differences in cytomorphological features on conventional cytology smears and liquid-based cytology preparations.

S. No.	Cytological diagnosis	Morphological features on conventional smears	Morphological features on liquid-based cytology
1.	Colloid Nodular Goitre	Thyroid follicular cells with colloid in the backgroundBlood in the background [Figure 3]	Clear background showing only scant colloid in small droplets, no blood in the background [Figure 4]
2.	Hashimoto’s thyroiditis/lymphocytic thyroiditis	Thyroid follicular cells with lymphocytic impingement. Blood in the background [Figure 1]	Clearer background [Figure 2]
3.	Neoplasms	Cytomorphological features and arrangements easily appreciated on Conventional Smears	Nuclear features are better appreciated on LBC. Easier to perform ancillary techniques like Immunocytochemistry and special stains to reach out a better and early diagnosis [Figures 5 and 6]