Literature DB >> 3592567

Determination of 4-hydroxy-3-methoxymandelic acid in urine using HPLC with electrochemical detection.

S M Palfrey.   

Abstract

A method is described for the assay of 4-hydroxy-3-methoxymandelic acid (HMMA) in urine using HPLC coupled with electrochemical detection. In this method acidified urine is extracted with ethyl acetate which is then washed with the chromatography solvent to back extract HMMA. The solvent is a pH 5.0 citrate buffer. At this pH, HMMA separates clearly from all other electroactive compounds present in urine.

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Year:  1987        PMID: 3592567     DOI: 10.1177/000456328702400208

Source DB:  PubMed          Journal:  Ann Clin Biochem        ISSN: 0004-5632            Impact factor:   2.057


  1 in total

Review 1.  Desktop laboratory technology in general practice.

Authors:  N C Stott
Journal:  BMJ       Date:  1989-09-02
  1 in total

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