| Literature DB >> 3592567 |
Abstract
A method is described for the assay of 4-hydroxy-3-methoxymandelic acid (HMMA) in urine using HPLC coupled with electrochemical detection. In this method acidified urine is extracted with ethyl acetate which is then washed with the chromatography solvent to back extract HMMA. The solvent is a pH 5.0 citrate buffer. At this pH, HMMA separates clearly from all other electroactive compounds present in urine.Entities:
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Year: 1987 PMID: 3592567 DOI: 10.1177/000456328702400208
Source DB: PubMed Journal: Ann Clin Biochem ISSN: 0004-5632 Impact factor: 2.057