| Literature DB >> 35916396 |
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Abstract
Entities:
Year: 2022 PMID: 35916396 PMCID: PMC9344821 DOI: 10.1111/jcmm.17487
Source DB: PubMed Journal: J Cell Mol Med ISSN: 1582-1838 Impact factor: 5.295
FIGURE 5DEK positively regulates the progression of EMT in human bronchial epithelial cell. (A) Flow cytometry analysis of TGF‐β1 expression in the BEAS‐2B cells with ovDEK or shDEK (right panel) and DEK expression after treating with TGF‐β1 (left panel). (B) Relative level of TGF‐β1 and DEK. (C) The DEK, α‐SMA, vimentin, E‐cadherin and Snail + Slug were detected after TGF‐β1 treatment using Western blotting. (D) Relative density of above proteins. (E) mRNA levels of α‐SMA, vimentin and E‐cadherin were detected. (F) Immunofluorescence was performed for analysis of E‐cadherin and vimentin in the BEAS‐2B cells. NC, negative control cells; ovDEK, cells transfected with DEK; shNC, cells transfected with scrambled shRNA; shDEK, cells transfected with DEK shRNA.*p < 0.05, vs control group. # p < 0.05, ovDEK vs TGF‐β1 + NC group; shDEK v sTGF‐β1 + shNC group.