Overexpression of BpERF1.1 in Betula Platyphylla enhanced tolerance to multiple abiotic stresses.

Cold, salt and drought are the main environmental stresses affecting plant growth and crop yield. ERF1, a member of the well-studied ERF gene family, has been proven to play an important role in abiotic stress in plants. In this study, an ERF1, BpERF1.1, was overexpressed in birch (Betula Platyphylla Suk.) and 11 transgenic lines were obtained. Effects of cold, salt and drought treatments were  checked  on the BpERF1.1 overexpressing lines and the corresponding WT line. The transgenic lines showed improved tolerance against these abiotic stresses than the WT line. Further RNA-seq analysis identified 689 differentially expressed genes (DEGs) in the transgenic birch compared with WT, including 228 up-regulated genes and 461 down-regulated genes. The result of gene ontology enrichment analysis showed that among these DEGs, 273 genes were involved in various plant biological process, and 83% of them were involved in cellular process, metabolic process, biological regulation and response to stimulus (11%). All these results demonstrate that BpERF1.1 gene improves the tolerance and resistance of birch against cold, salt and drought stress, probably by interconnecting with other genes involved in plant response to abiotic stresses. Supplementary Information: The online version contains supplementary material available at 10.1007/s12298-022-01206-3. © Prof. H.S. Srivastava Foundation for Science and Society 2022.