Natália Medeiros Dias Lopes1, Hannah Hamada Mendonça Lens1, Walison Augusto da Silva Brito1,2, Julya Karen Bianchi1, Poliana Camila Marinello1,3, Rubens Cecchini4, André Armani5, Alessandra Lourenço Cecchini6. 1. Laboratory of Molecular Pathology, Universidade Estadual de Londrina, Celso Garcia Cid, PR445, Km 380 University Campus, Londrina, Paraná, CEP 86057-970, Brazil. 2. Leibniz-Institute for Plasma Science and Technology (INP Greifswald), ZIK Plasmatis "Plasma Redox Effects", Greifswald, Germany. 3. Department of Radiation Physics, The University of Texas MD Anderson Cancer Center, Houston, TX, USA. 4. Laboratory of Physiopathology and Free Radicals, Universidade Estadual de Londrina, Londrina, PR, Brazil. 5. Department of Surgery, Universidade Estadual de Londrina, Londrina, PR, Brazil. 6. Laboratory of Molecular Pathology, Universidade Estadual de Londrina, Celso Garcia Cid, PR445, Km 380 University Campus, Londrina, Paraná, CEP 86057-970, Brazil. alcecchini@uel.br.
Abstract
PURPOSE: Papillary thyroid carcinoma (PTC) is the most frequent subtype of thyroid cancer; Hashimoto's thyroiditis (HT), autoimmune disease, commonly affects the thyroid gland; there is possibly a correlation between both, but the exact mechanisms that involve this relationship are still under debate. Since oxidative stress (OS) and the inflammatory environment participate in the development of several types of cancer, the objective of the present study was to establish the microenvironment and systemic participation of OS and inflammatory markers in patients with PTC and HT. METHODS: Blood and tissue samples were collected from 115 patients: BENIGN (n = 63); PTC (n = 27); HT (n = 15) and PTC + HT (n = 10), and sixty-three were samples from healthy individuals (control group). RESULTS: Superoxide dismutase, Catalase, reduced Glutathione, markers of lipid peroxidation and inflammation were evaluated in blood. Immunohistochemistry was performed on 3-nitrotyrosine, 4-hydroxynonenal, Ki-67 and VEGF. The results indicate that antioxidant enzymes were more active in groups with thyroid disorders compared to control, while the concentration of Reduced glutathione was reduced in BENIGN and PTC groups. When PTC and PTC + HT groups were analyzed, no significant differences were found in relation to the antioxidant defense and inflammatory markers. The ability to contain the induced lipid peroxidation was lower and a high level of malondialdehyde was observed in the PTC group. All immunohistochemical markers had higher scores in the PTC group compared to PTC + HT. CONCLUSION: There was a more pronounced presence of OS and a greater activity of cell proliferation and angiogenesis markers in PTC than in PTC + HT group.
PURPOSE: Papillary thyroid carcinoma (PTC) is the most frequent subtype of thyroid cancer; Hashimoto's thyroiditis (HT), autoimmune disease, commonly affects the thyroid gland; there is possibly a correlation between both, but the exact mechanisms that involve this relationship are still under debate. Since oxidative stress (OS) and the inflammatory environment participate in the development of several types of cancer, the objective of the present study was to establish the microenvironment and systemic participation of OS and inflammatory markers in patients with PTC and HT. METHODS: Blood and tissue samples were collected from 115 patients: BENIGN (n = 63); PTC (n = 27); HT (n = 15) and PTC + HT (n = 10), and sixty-three were samples from healthy individuals (control group). RESULTS: Superoxide dismutase, Catalase, reduced Glutathione, markers of lipid peroxidation and inflammation were evaluated in blood. Immunohistochemistry was performed on 3-nitrotyrosine, 4-hydroxynonenal, Ki-67 and VEGF. The results indicate that antioxidant enzymes were more active in groups with thyroid disorders compared to control, while the concentration of Reduced glutathione was reduced in BENIGN and PTC groups. When PTC and PTC + HT groups were analyzed, no significant differences were found in relation to the antioxidant defense and inflammatory markers. The ability to contain the induced lipid peroxidation was lower and a high level of malondialdehyde was observed in the PTC group. All immunohistochemical markers had higher scores in the PTC group compared to PTC + HT. CONCLUSION: There was a more pronounced presence of OS and a greater activity of cell proliferation and angiogenesis markers in PTC than in PTC + HT group.
Authors: Csaba Molnár; Sarolta Molnár; Judit Bedekovics; Attila Mokánszki; Ferenc Győry; Endre Nagy; Gábor Méhes Journal: Pathol Oncol Res Date: 2019-01-21 Impact factor: 3.201