| Literature DB >> 35895273 |
François Halloy1,2, Paulina Brönnimann1,3, Jonathan Hall1, Daniel Schümperli4.
Abstract
We describe methods to follow the fate of oligonucleotides after their injection into experimental animals. The quantitation in various tissues, blood or bone marrow cells is possible by chemical ligation PCR. This method works independently of chemical modifications of the oligonucleotide and/or its conjugations to lipid or peptide moieties. Moreover, metabolization intermediates can be detected by mass spectrometry. Together with a readout assay for the biochemical or physiological effects, which will differ, depending on the particular purpose of the oligonucleotide, these methods allow for a comprehensive understanding of oligonucleotide behavior in a living organism.Entities:
Keywords: Chemical ligation qPCR; Mass spectrometry; Oligonucleotide biodistribution; Oligonucleotide conjugates; Oligonucleotide therapeutics
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Year: 2022 PMID： 35895273 DOI： 10.1007/978-1-0716-2521-7_19
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745