Literature DB >> 35893604

Developing Oligo Probes for Chromosomes Identification in Hemp (Cannabis sativa L.).

Dmitry V Romanov1, Gennady I Karlov1, Mikhail G Divashuk1.   

Abstract

Hemp (Cannabis sativa L., 2n = 20) is a valuable crop that is successfully used as a food, technical and medicinal crop. It is a dioecious plant with an XX\XY sex determination system. Some chromosomes of C. sativa have almost the same lengths and centromeric indexes. Cytogenetic markers help to distinguish similar plant chromosomes, including sex chromosomes, which is important for the breeding process. Two repeats (CS-1 and CS-237) were used to develop labeled oligo-probes for rapid and low-cost oligo-FISH. These oligos can be recommended for use as cytological markers to distinguish sex chromosomes (X and Y) and somatic chromosome pairs 3, 6, and 8 by rapid oligo-FISH in a short time.

Entities:  

Keywords:  crop; cytogenetic markers; dioecious; hybridization; oligo-FISH; repeat

Year:  2022        PMID： 35893604      PMCID： PMC9332022          DOI： 10.3390/plants11151900

Source DB:  PubMed          Journal:  Plants (Basel)        ISSN： 2223-7747


  41 in total

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5.  Study and Physical Mapping of the Species-Specific Tandem Repeat CS-237 Linked with 45S Ribosomal DNA Intergenic Spacer in Cannabis sativa L.

Authors:  Oleg S Alexandrov; Dmitry V Romanov; Mikhail G Divashuk; Olga V Razumova; Daniil S Ulyanov; Gennady I Karlov
Journal:  Plants (Basel)       Date:  2022-05-24

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Journal:  Plant Biotechnol J       Date:  2018-05-23       Impact factor: 9.803

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10.  Physical mapping of repetitive oligonucleotides facilitates the establishment of a genome map-based karyotype to identify chromosomal variations in peanut.

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Journal:  BMC Plant Biol       Date:  2021-02-20       Impact factor: 4.215
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