| Literature DB >> 35881385 |
Tonia Woodberry1, Jessica Rita Loughland1,2, Gabriela Minigo3,4.
Abstract
This protocol outlines a method for the timely detection of intracellular cytokines produced by activated dendritic cells (DC) in human whole blood. The quantification of cytokines is used to measure DC immune responsiveness, providing information on the breadth, strength, and DC subtypes responding spontaneously and to specific stimulation with toll-like receptor (TLR) ligands or parasite-infected erythrocytes. DC subsets, plasmacytoid DC, CD1c+ DC, CD141+ DC, and CD16+ DC, are examined in their natural environment of plasma and blood cells (erythrocytes, neutrophils, platelets, and leukocytes) enabling disease, medication, nutritional, and hematological effects on DC function to be examined in vaccine studies, ageing, health, and disease.Entities:
Keywords: Blood dendritic cell antigen (BDCA); Dendritic cell subsets; Human assay; Intracellular cytokine production; Malaria; Parasite; Toll-like receptor; Whole blood
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Year: 2022 PMID： 35881385 DOI： 10.1007/978-1-0716-2189-9_53
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745