Literature DB >> 35881378

Antibody-Dependent THP-1 Cell-Mediated Phagocytosis of Plasmodium falciparum-Infected Erythrocytes.

Meseret Workineh Kassa1, Wina Hasang1, Stephen John Rogerson2.   

Abstract

Antibodies that recognize variant surface antigens (VSAs) expressed on Plasmodium falciparum-infected erythrocytes (IEs) opsonize IEs for phagocytic clearance. The anti-VSA antibodies promote antibody-dependent cellular phagocytosis (ADCP) of IEs by interacting with innate immune cells. ADCP is an important immune effector mechanism of parasite clearance. ADCP can be a tool to assess the efficacy of vaccine-induced antibodies, in addition to measuring the neutralizing ability of antibodies. Here, we developed and validated an efficient and high-throughput plate-based flow cytometric assay to measure ADCP of IEs using the human monocytic THP-1 cell line. This flow cytometric assay can be used to analyze the level of naturally acquired or vaccine-induced opsonic antibodies in large cohorts.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Antibodies; Infected erythrocytes; Malaria; Phagocytosis; THP-1 cells; Variant surface antigens

Mesh：

Substances：

Year:  2022        PMID： 35881378     DOI： 10.1007/978-1-0716-2189-9_46

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  5 in total

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Journal:  Lancet Infect Dis       Date:  2020-02-04       Impact factor: 25.071

2.  Human cerebral malaria. A quantitative ultrastructural analysis of parasitized erythrocyte sequestration.

Authors:  G G MacPherson; M J Warrell; N J White; S Looareesuwan; D A Warrell
Journal:  Am J Pathol       Date:  1985-06       Impact factor: 4.307

3.  Antibodies that induce phagocytosis of malaria infected erythrocytes: effect of HIV infection and correlation with clinical outcomes.

Authors:  Ricardo Ataíde; Victor Mwapasa; Malcolm E Molyneux; Steven R Meshnick; Stephen J Rogerson
Journal:  PLoS One       Date:  2011-07-19       Impact factor: 3.240

4.  High throughput functional assays of the variant antigen PfEMP1 reveal a single domain in the 3D7 Plasmodium falciparum genome that binds ICAM1 with high affinity and is targeted by naturally acquired neutralizing antibodies.

Authors:  Andrew V Oleinikov; Emily Amos; Isaac Tyler Frye; Eddie Rossnagle; Theonest K Mutabingwa; Michal Fried; Patrick E Duffy
Journal:  PLoS Pathog       Date:  2009-04-17       Impact factor: 6.823

5.  Concentration and purification by magnetic separation of the erythrocytic stages of all human Plasmodium species.

Authors:  Clotilde Ribaut; Antoine Berry; Séverine Chevalley; Karine Reybier; Isabelle Morlais; Daniel Parzy; Françoise Nepveu; Françoise Benoit-Vical; Alexis Valentin
Journal:  Malar J       Date:  2008-03-05       Impact factor: 2.979
  5 in total

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