Literature DB >> 35881360

Production of anti-PfEMP1 Polyclonal Antisera in Rats and Mice.

Rebecca W Olsen1, Jennifer Suurbaar2,3,4, Anja Ramstedt Jensen2.   

Abstract

Plasmodium falciparum-infected erythrocytes (IEs) bind various host receptors via members of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) family expressed on the surface of the IEs. Antibody reagents are needed to investigate interactions between specific PfEMP1 proteins and receptors expressed by human endothelial cells. This protocol describes the production of rat and mouse polyclonal anti-PfEMP1 antibodies. Polyclonal antibodies are relatively easy to produce and have advantages compared to monoclonal antibodies (see Chapters 28 - 30 ) for some applications. An ELISA-based method to test the polyclonal antibodies before their use in more advanced procedures is also presented.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Adhesion; Antisera; Immunization; Malaria; Mice; PfEMP1; Plasmodium falciparum; Polyclonal antibodies; Protein antigen; Rats

Mesh：

Substances：

Year:  2022        PMID： 35881360     DOI： 10.1007/978-1-0716-2189-9_28

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  4 in total

1.  Plasmodium falciparum erythrocyte membrane protein 1 domain cassettes 8 and 13 are associated with severe malaria in children.

Authors:  Thomas Lavstsen; Louise Turner; Fredy Saguti; Pamela Magistrado; Thomas S Rask; Jakob S Jespersen; Christian W Wang; Sanne S Berger; Vito Baraka; Andrea M Marquard; Andaine Seguin-Orlando; Eske Willerslev; M Thomas P Gilbert; John Lusingu; Thor G Theander
Journal:  Proc Natl Acad Sci U S A       Date:  2012-05-22       Impact factor: 11.205

2.  Immunization with Recombinant Plasmodium falciparum Erythrocyte Membrane Protein 1 CIDRα1 Domains Induces Domain Subtype Inhibitory Antibodies.

Authors:  Louise Turner; Thor G Theander; Thomas Lavstsen
Journal:  Infect Immun       Date:  2018-10-25       Impact factor: 3.441

3.  Surface co-expression of two different PfEMP1 antigens on single plasmodium falciparum-infected erythrocytes facilitates binding to ICAM1 and PECAM1.

Authors:  Louise Joergensen; Dominique C Bengtsson; Anja Bengtsson; Elena Ronander; Sanne S Berger; Louise Turner; Michael B Dalgaard; Gerald K K Cham; Michala E Victor; Thomas Lavstsen; Thor G Theander; David E Arnot; Anja T R Jensen
Journal:  PLoS Pathog       Date:  2010-09-02       Impact factor: 6.823

4.  Natural and Vaccine-Induced Acquisition of Cross-Reactive IgG-Inhibiting ICAM-1-Specific Binding of a Plasmodium falciparum PfEMP1 Subtype Associated Specifically with Cerebral Malaria.

Authors:  Rebecca W Olsen; Gertrude Ecklu-Mensah; Anja Bengtsson; Michael F Ofori; John P A Lusingu; Filip C Castberg; Lars Hviid; Yvonne Adams; Anja T R Jensen
Journal:  Infect Immun       Date:  2018-03-22       Impact factor: 3.441
  4 in total

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