Natália Helena Gavilan1, Lucas Junqueira de Freitas Morel2, Juliana da Silva Coppede2, Silvia Helena Taleb-Contini2, Suzelei de Castro França2, Bianca Waléria Bertoni2,3, Ana Maria Soares Pereira4,5. 1. Departamento de Horticultura, Universidade Estadual Paulista "Júlio de Mesquita Filho", Botucatu, São Paulo, 18618-970, Brazil. 2. Departamento de Biotecnologia em Plantas Medicinais, Universidade de Ribeirão Preto-UNAERP, Ribeirão Preto, São Paulo, 14096-900, Brazil. 3. Jardim Botânico de Plantas Medicinais Ordem e Progresso, Jardinópolis, São Paulo, 14680-000, Brazil. 4. Departamento de Biotecnologia em Plantas Medicinais, Universidade de Ribeirão Preto-UNAERP, Ribeirão Preto, São Paulo, 14096-900, Brazil. apereira@unaerp.br. 5. Jardim Botânico de Plantas Medicinais Ordem e Progresso, Jardinópolis, São Paulo, 14680-000, Brazil. apereira@unaerp.br.
Abstract
BACKGROUND: Pyrostegia venusta (Ker Gawl.) Miers occurs in threatened biodiversity hotspots of Cerrado and Atlantic forest biomes in Brazil and is used in traditional medicine to treat various respiratory and skin diseases. METHODS AND RESULTS: This study (i) examined the genetic diversity and structure of six natural populations of P. venusta from different Brazilian regions using sequence-related amplified polymorphism (SRAP) markers; and (ii) compared the intra- and inter-populational levels of the bioactive component verbascoside using high-performance liquid chromatography. The population from Nova Mutum, Mato Grosso, presented the highest genetic variability (Nei index H = 0.2759; Shannon index I = 0.4170; 85.14% polymorphic loci), whereas the population from Araxá, Minas Gerais, presented the lowest genetic variability (H = 0.1811; I = 0.2820; 70.27% polymorphic loci). The intra-populational variability (79%) was significantly higher (p = 0.001) than the inter-populational variability (21%). The populations were clustered into two groups but their genetic differentiation was not associated with geographical origin (Mantel test, r = 0.328; p > 0.05). The verbascoside content significantly differed (p > 0.05) among the six populations and between the individuals from each population. The highest verbascoside levels (> 200 µg/mg extract) were detected in populations from Araxá and Serrana, while the lowest verbacoside levels were detected in populations from Paranaíta and Sinop. CONCLUSIONS: This is the first report on the use of SRAP markers to analyze genetic variability in the family Bignoniaceae. Our findings shall help to better understand the genetic and chemical diversity of P. venusta populations, as well as provide useful information to select the most appropriate individuals to prepare phytomedicines.
BACKGROUND: Pyrostegia venusta (Ker Gawl.) Miers occurs in threatened biodiversity hotspots of Cerrado and Atlantic forest biomes in Brazil and is used in traditional medicine to treat various respiratory and skin diseases. METHODS AND RESULTS: This study (i) examined the genetic diversity and structure of six natural populations of P. venusta from different Brazilian regions using sequence-related amplified polymorphism (SRAP) markers; and (ii) compared the intra- and inter-populational levels of the bioactive component verbascoside using high-performance liquid chromatography. The population from Nova Mutum, Mato Grosso, presented the highest genetic variability (Nei index H = 0.2759; Shannon index I = 0.4170; 85.14% polymorphic loci), whereas the population from Araxá, Minas Gerais, presented the lowest genetic variability (H = 0.1811; I = 0.2820; 70.27% polymorphic loci). The intra-populational variability (79%) was significantly higher (p = 0.001) than the inter-populational variability (21%). The populations were clustered into two groups but their genetic differentiation was not associated with geographical origin (Mantel test, r = 0.328; p > 0.05). The verbascoside content significantly differed (p > 0.05) among the six populations and between the individuals from each population. The highest verbascoside levels (> 200 µg/mg extract) were detected in populations from Araxá and Serrana, while the lowest verbacoside levels were detected in populations from Paranaíta and Sinop. CONCLUSIONS: This is the first report on the use of SRAP markers to analyze genetic variability in the family Bignoniaceae. Our findings shall help to better understand the genetic and chemical diversity of P. venusta populations, as well as provide useful information to select the most appropriate individuals to prepare phytomedicines.
Authors: Ana Maria S Pereira; Camila Hernandes; Sarazete I V Pereira; Bianca W Bertoni; Suzelei C França; Paulo S Pereira; Silvia H Taleb-Contini Journal: Chem Biol Interact Date: 2014-10-29 Impact factor: 5.192
Authors: Andiamira Cagnoni Balestra; Camila Mira Sandy; Fernando Ramalho; Alceu Afonso Jordão Júnior; Silvia Helena Taleb Contini; Eduardo José Crevelin; Fabio Carmona; Ana Maria S Pereira; Marcos C Borges Journal: J Asthma Date: 2020-02-24 Impact factor: 2.515
Authors: Isabela Cristina G Honório; Juliana S Coppede; Piero G Delprete; Frederico Henrique S Costa; Mariana P C Telles; Ramilla S Braga; José Alexandre F Diniz-Filho; Valéria S C Correa; Suzelei C França; Ana Maria S Pereira; Bianca Waleria Bertoni Journal: PLoS One Date: 2018-10-26 Impact factor: 3.240