| Literature DB >> 358585 |
H Adachi, T Fukuda, S Funahashi, T Kurahori, E Ishikawa.
Abstract
A sandwich enzymoimmunoassay (EIA) procedure was developed for the detection of HBsAg using Fab' of anti-HBsAg conjugated with beta-D-galactosidase from Escherichia coli with anti-HBsAg-coated silicone rubber discs as a solid phase. EIA could detect 1 ng/ml of HBsAg. It was as sensitive as radioimmunoassay (RIA, AusRia II) and about 30-fold more sensitive than reversed passive hemagglutination assay RPHA, ReverseCell). EIA and RIA could detect more HBsAg-positive sera than RPHA.Entities:
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Year: 1978 PMID： 358585 DOI： 10.1111/j.1423-0410.1978.tb02925.x
Source DB: PubMed Journal: Vox Sang ISSN： 0042-9007 Impact factor: 2.144