Kimia Kiani-Azad1,2, Masoumeh Bagheri3, Mostafa Sadeghi2, Shahla Nematollahian4, Azadeh Zahmatkesh1, Mojtaba Moharrami1, Seyed Reza Miraie-Ashtiani2. 1. Razi Vaccine and Serum Research Institute (RVSRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, 31976-19751, Iran. 2. Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran. 3. Razi Vaccine and Serum Research Institute (RVSRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, 31976-19751, Iran. m.bagheri@rvsri.ac.ir. 4. Iran Silkworm Research Center (ISRC) Agricultural Research, Education and Extension Organization (AREEO), Rasht, Iran.
Abstract
PURPOSE: Pebrine as the most dangerous disease of silkworm mostly caused by Nosema species has caused huge economic losses. There is no information on the species and the genomic sequences of the pebrine-causing microsporidia in Iran. METHODS: In the present research, we tried to determine the sequences of two regions of rDNA using molecular methods. First, infected larvae and mother moths were collected from several farms in the north of Iran for identification and molecular characterization of microsporidian isolates. After extracting the spores and genomic DNA from the collected samples, two fragments of internal transcribed spacer (ITS) rDNA and small subunit (SSU) rDNA were amplified and sequenced, and registered in NCBI database and then, the phylogenetic tree was drawn. RESULTS: Results showed the obtained sequences (ITS rDNA: Accession No. MZ322002 and SSU rDNA: Accession No. MZ314703) represent a new strain of Nosema bombycis, which differs from the sequences deposited in the NCBI. CONCLUSION: The new N. bombycis strain identified in our study will help in control and management of the pebrine disease by specific detection of the infectious agent.
PURPOSE: Pebrine as the most dangerous disease of silkworm mostly caused by Nosema species has caused huge economic losses. There is no information on the species and the genomic sequences of the pebrine-causing microsporidia in Iran. METHODS: In the present research, we tried to determine the sequences of two regions of rDNA using molecular methods. First, infected larvae and mother moths were collected from several farms in the north of Iran for identification and molecular characterization of microsporidian isolates. After extracting the spores and genomic DNA from the collected samples, two fragments of internal transcribed spacer (ITS) rDNA and small subunit (SSU) rDNA were amplified and sequenced, and registered in NCBI database and then, the phylogenetic tree was drawn. RESULTS: Results showed the obtained sequences (ITS rDNA: Accession No. MZ322002 and SSU rDNA: Accession No. MZ314703) represent a new strain of Nosema bombycis, which differs from the sequences deposited in the NCBI. CONCLUSION: The new N. bombycis strain identified in our study will help in control and management of the pebrine disease by specific detection of the infectious agent.
Authors: G Subrahmanyam; Vijaya Gowri Esvaran; Kangayam Muthusamy Ponnuvel; W Hassan; M Chutia; R Das Journal: Indian J Microbiol Date: 2019-08-28 Impact factor: 2.461