| Literature DB >> 35857237 |
Rouhollah Habibey1, Johannes Striebel1, Kritika Sharma1, Volker Busskamp2.
Abstract
Spontaneous and optogenetically evoked activities of human induced pluripotent stem cell (hiPSC)-derived neurons can be assessed by patch clamp and multi-electrode array (MEA) electrophysiology. Optogenetic activation of these human neurons facilitates the characterization of their functional properties at the single neuron and circuit level. Here we showcase the preparation of hiPSC-derived neurons expressing optogenetic actuators, in vitro optogenetic stimulation and simultaneous functional recordings using patch clamp and MEA electrophysiology.Entities:
Keywords: Banker culture; Induced pluripotent stem cells (iPSCs); Long-term iPSC-derived neuronal culture; Multi-electrode array (MEA) electrophysiology; Neuron-astrocyte co-culture; Optogenetic stimulation; Patch clamp
Mesh：
Year: 2022 PMID： 35857237 DOI： 10.1007/978-1-0716-2329-9_17
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745