Inhibition of KIR2.1 decreases pulmonary artery smooth muscle cell proliferation and migration.

The investigation of effective therapeutic drugs for pulmonary hypertension (PH) is critical. KIR2.1 plays crucial roles in regulating cell proliferation and migration, and vascular remodeling. However, researchers have not yet clearly determined whether KIR2.1 participates in the proliferation and migration of pulmonary artery smooth muscle cells (PASMCs) and its role in pulmonary vascular remodeling (PVR) also remains elusive. The present study aimed to examine whether KIR2.1 alters PASMC proliferation and migration, and participates in PVR, as well as to explore its mechanisms of action. For the in vivo experiment, a PH model was established by intraperitoneally injecting Sprague‑Dawley rats monocrotaline (MCT). Hematoxylin and eosin staining revealed evidence of PVR in the rats with PH. Immunofluorescence staining and western blot analysis revealed increased levels of the KIR2.1, osteopontin (OPN) and proliferating cell nuclear antigen (PCNA) proteins in pulmonary blood vessels and lung tissues following exposure to MCT, and the TGF‑β1/SMAD2/3 signaling pathway was activated. For the in vitro experiments, the KIR2.1 inhibitor, ML133, or the TGF‑β1/SMAD2/3 signaling pathway blocker, SB431542, were used to pre‑treat human PASMCs (HPASMCs) for 24 h, and the cells were then treated with platelet‑derived growth factor (PDGF)‑BB for 24 h. Scratch and Transwell assays revealed that PDGF‑BB promoted cell proliferation and migration. Immunofluorescence staining and western blot analysis demonstrated that PDGF‑BB upregulated OPN and PCNA expression, and activated the TGF‑β1/SMAD2/3 signaling pathway. ML133 reversed the proliferation and migration induced by PDGF‑BB, inhibited the expression of OPN and PCNA, inhibited the TGF‑β1/SMAD2/3 signaling pathway, and reduced the proliferation and migration of HPASMCs. SB431542 pre‑treatment also reduced cell proliferation and migration; however, it did not affect KIR2.1 expression. On the whole, the results of the present study demonstrate that KIR2.1 regulates the TGF‑β1/SMAD2/3 signaling pathway and the expression of OPN and PCNA proteins, thereby regulating the proliferation and migration of PASMCs and participating in PVR.

Introduction

Pulmonary hypertension (PH) is a cardiopulmonary vascular disease characterized by continued increases in pulmonary arterial pressure and pulmonary vascular resistance (1). Current treatment strategies mainly focus on reducing pulmonary vascular resistance and increasing blood flow. However, these approaches are not effective for a long period of time. Therefore, the identification of a precise therapeutic target for PH is critical. The hallmark feature of PH is medial pulmonary artery hyperplasia, which is mainly caused by the abnormal proliferation and aggregation of pulmonary artery smooth muscle cells (PASMCs) (2,3). Therefore, the proliferation, migration, apoptosis and extracellular matrix deposition of PASMCs are critical targets for studying PH.

A number of growth factors are related to PH and vascular remodeling, including platelet-derived growth factor (PDGF)-BB and transforming growth factor (TGF)-β (4). The inhibition of the PDGF receptor has been shown to increase the survival rate of rats with monocrotaline (MCT)-induced PH (5,6). The TGF-β superfamily contains a large number of cytokine growth factors that control numerous cellular functions, including proliferation, migration, differentiation, and extracellular matrix secretion and deposition (4). It has been reported that PDGF-BB activates the TGF-β1/SMAD2/3 signaling pathway, and induces the growth and proliferation of rat PASMCs (7). The TGF-β1/SMAD pathway is activated in animals with MCT and hypoxia-induced PH (8-10) and in patients with PH (11). The activation of the TGF-β1/SMAD2/3 signaling pathway is one of the factors contributing to the occurrence and development of PH.

Inwardly rectifying K+ channel (KIR)2.1 encoded by the KCNJ2 gene is a member of the classical inwardly rectifying K+ channel family (KIR2 subfamily). In previous research, KIR2.1 was considered the main component of the inward rectifying potassium current of the heart and an essential component of the stable resting membrane potential of cardiomyocytes (12). However, KIR2.1 is related to cell proliferation and migration (13-17) and vascular remodeling (18). According to previous studies, KIR2.1 is expressed in isolated rat basilar artery, coronary artery, mesenteric artery smooth muscle cells (19), renal arteriole smooth muscle cells (20) and PASMCs (21). KIR2.1 gene knockout significantly inhibits the proliferation and migration of rat vascular smooth muscle cells (VSMCs) stimulated by PDGF-BB (22). However, researchers have not yet determined whether KIR2.1 participates in the proliferation and migration of PASMCs.

Therefore, the present study aimed to examine the effects of KIR2.1 inhibition on the proliferation and migration of PASMCs induced by PDGF-BB. In addition, the present study examined whether KIR2.1 regulates the TGF-β1/Smad2/3 signaling pathway by maintaining the depolarized membrane potential of the cell membrane, and whether it regulates the proliferation and migration of PASMCs.

Materials and methods

Animal model and treatment strategy

A total of 12 Sprague-Dawley (SD) rats (8-10 weeks old, weighing 200-250 g) were used in the experiments. The rats were purchased from Beijing Vital River Experimental Animal Co., Ltd. (license no. SCXK Beijing 2016-0006). The animals were maintained in environmentally controlled conditions (adequate cage size; free access to food and water; temperature, 22±2°C; humidity, 50-55%) on a 12-h light/12-h dark cycle. All procedures involving animals were performed in accordance with ethical standards and approved by the Institutional Animal Care and Use Committee of the Affiliated Hospital of Shihezi University School of Medicine (approval no. A 2020-165-01). Applicable guidelines were followed in accordance with the 'Guide for the Care and Use' published by the American Physiological Society (23).

A total of 12 rats were randomly and equally divided into two groups as follows: The control (CON) group and MCT group [60 mg/kg MCT (24-26) administered by intraperitoneal (i.p.) injection on the first day; Sigma-Aldrich; Merck KGaA].

Doppler echocardiography measurements

The Doppler echo parameter, pulmonary artery acceleration time (PAAT) is considered an echocardiographic indicator of PH (27). PAAT is the time interval between the onset of systolic pulmonary arterial flow and peak flow velocity. Previous studies have demonstrated that PAAT is inversely proportional to pulmonary vascular resistance (28-30). If the pulmonary vascular resistance increases, the pulmonary artery pressure also increases. Doppler echocardiography was used to assess PH on the 28th day following model establishment in the SD rats. A transthoracic closed-chest echocardiography was performed using a Vivid E9 ultrasound system equipped with a 12-MHz transducer (GE Healthcare; Cytiva). The rats were anesthetized by administering an i.p. injection of 3% sodium pentobarbital (40 mg/kg). PAAT was measured near the pulmonary valve on the left side of the chest. EchoPAC™ BT11 software (v.6.5; GE Healthcare; Cytiva) was used to analyze the data.

Right ventricular hypertrophy measurement

For the measurement of right ventricular hypertrophy, the rats were euthanized by an i.p. injection of 3% sodium pentobarbital (100 mg/kg) combined with CO2 anesthesia at a displacement of 70% vol/min. The heart tissue was collected and weighed to evaluate the right ventricular hypertrophy index (RVHI). The atrium and external blood vessels were separated from the isolated heart in 0.9% normal saline. The weights of both the right ventricle (RV) and left ventricle (LV) plus septum (S) were recorded. The RVHI was calculated using the formula: [RV/(LV + S)].

Histopathological examination of lung tissue

Lung tissues were harvested, fixed in 4% paraformaldehyde (Boster Co., Ltd.), dehydrated, cleared, waxed, embedded, sectioned, patched and cut into slices (4-µm-thick). Hematoxylin and eosin (H&E) staining or Masson's trichome staining (Beijing Solarbio Biotechnology Co., Ltd.) were used to evaluate the pulmonary artery morphology. Lung tissues were observed and photographed using a digital camera (BX51; Olympus Corporation). Image-Pro Plus v.6.0 software (Media Cybernetics, Inc.) was used for the quantitative analysis. Pulmonary vascular remodeling (PVR) was evaluated by calculating the percentage of the thickness of the vessel wall (WT%) and the percentage of the vessel wall area (WA%): WT%=[2× (blood vessel outer diameter-blood vessel inner diameter)]/(blood vessel outer diameter) ×100%; WA%=(total area of blood vessel-blood vessel internal area)/total area of blood vessel ×100% (31). Two professional pathologists randomly selected 20 different non-overlapping fields from each section and analyzed PVR and lung fibrosis with Image-Pro Plus v.6.0 software (Media Cybernetics, Inc.). The pulmonary fibrosis index was analyzed by calculating the ratio of the total area of collagen to the total area of connective tissue in each field of view, as previously described (32).

Cell culture and treatment

Human PASMCs (HPASMCs) (Shanghai iCell Bioscience Inc.) were cultured in high-glucose DMEM (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (Gibco; Thermo Fisher Scientific, Inc.). The cells were incubated at 37°C for 24 h in a humidified 5% CO2 atmosphere.

For the cell treatments, the cells were first pre-treated with the KIR2.1 pathway blocker, ML133 (20 µM, ApexBio) (14,17,33), or the TGF-β1/SMAD signaling pathway blocker, SB431542 (10 µM, ApexBio), for 24 h and were then treated with PDGF-BB (25 ng/ml, PeproTech, Inc.) (34-36) for 24 h. Cells in the control group were not treated. The experiment was repeated six times (n=6).

Cell scratch assay

HPASMCs in the logarithmic growth phase were plated as monolayers on six-well plates and cultured at 37°C with 5% CO2 until the cell density reached 80%. A cell-free band was uniformly generated at the center of each well using a 1-ml pipet tip. The cells were washed twice with PBS and then incubated with 2 ml 2% DMEM/F12 low-serum medium and pretreated with 0 µM ML133 for 24 h, then treated with 25 ng/ml PDGF-BB for 24 h. Images were recorded and assessed at 0 and 24 h using an Olympus inverted microscope (Olympus Corporation). The migration distance was estimated using ImageJ v1.8.0 software (National Institutes of Health). The cell migration rate (%)=[(0 h average scratch area-24 h average scratch area)/0 h average scratch area] ×100% (37-39).

Transwell assay

The Transwell™ chamber (Corning, Inc.) was placed in a 24-well culture plate. Subsequently, 200 µl of the cell suspension (cell density of 104 cells/ml) were inoculated into the upper chamber. Complete DMEM/F12 containing 10% FBS (600 µl) was then added to the lower chamber. 20 µM ML133 was added for 24 h after the cells adhered to the well, then treated with 25 ng/ml PDGF-BB for 24 h. Following the intervention, the cells were cultured with fresh medium at 37°C in a humidified atmosphere containing 5% CO2 for 24 h. Subsequently, the cells below the membrane were fixed with 4% paraformaldehyde for 10 min at room temperature and stained with 0.1% crystal violet for 30 min at room temperature. A cotton swab was used to gently remove the cells from the upper surface of the chamber. Following observation with a light microscope and imaging with a digital camera (BX51, Olympus Corporation), five different fields of view were randomly selected; the cells that invaded the submembrane surface were counted, and the number of invaded cells reflected the strength of the invasive ability of the HPASMCs (37-39).

Immunofluorescence staining

The paraffin-embedded tissue sections were dewaxed, and antigen retrieval was performed. The cells were seeded on six-well glass slides for fixation. Triton X (0.3%) was used to permeabilize the membrane. The cells were then incubated overnight at 4°C with the following antibodies: Anti-KIR2.1 (1:100 dilution, cat. no. ab109750), anti-osteopontin (OPN; 1:500 dilution, cat. no. ab8448), anti-proliferating cell nuclear antigen (PCNA; 1:200 dilution, cat. no. ab29) and anti-α-smooth muscle actin (α-SMA; 1: 500; cat. no. ab124964) (all from Abcam). The following day, the cells were incubated with FITC-labeled goat anti-rabbit IgG (1:100; cat. no. ZF-0311) or TRITC-labeled anti-mouse IgG (1:100; ZF-0313) (Beijing Zhongshan Jinqiao Biotechnology Co., Ltd.) antibodies in a dark box at 37°C for 2 h. The nuclei were stained with DAPI (1:1,000, D9542; Sigma-Aldrich; Merck KGaA) at 37°C in a dark box for 20 min. The cells were observed and photographed under a fluorescence inverted microscope (LSM710; Carl Zeiss AG). Proteins were semi-quantitatively analyzed using Image-Pro Plus 6.0 software (National Institutes of Health).

Western blot analysis

Total protein was extracted from HPASMC suspensions or pulmonary vascular tissue homogenates from SD rats with RIPA Lysis Buffer (Thermo Fisher Scientific, Inc.) in western blot analysis. The BCA protein determination method was used to determine the protein concentration. Proteins aliquots (40 µg/lane) were separated using standard sodium lauryl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE, (8-10% gel)) and transferred to 0.45 µm nitrocellulose membranes (Invitrogen; Thermo Fisher Scientific, Inc.). The membranes were incubated overnight at 4°C with the following primary antibodies: Anti-GAPDH (1:10,000 dilution, cat. no. ab8245), anti-KIR2.1 (1:1,000 dilution, cat. no. ab109750), anti-TGF-β1 (1:1,000 dilution, cat. no. ab92486), anti-SMAD2 (1:1,000 dilution, cat. no. ab40855), anti-SMAD3 (1:1,000 dilution, cat. no. ab40854), anti-phosphorylated (p-)SMAD2 (1:1,000 dilution, cat. no. ab188334), anti-p-SMAD3 (1:1,000 dilution, cat. no. ab52903), anti-OPN (1:1,000 dilution, cat. no. ab8448) and anti-PCNA (1:1,000 dilution, cat. no. ab29) (all from Abcam). Subsequently, the membranes were incubated with HRP-labeled anti-mouse IgG (1:20,000 dilution; cat. no. ZB-2305) or anti-rabbit IgG (1:10,000 dilution; cat. no. ZB-5301; Beijing Zhongshan Jinqiao Biotechnology Co., Ltd.) at room temperature for 2 h. Luminescence reagents were obtained from the SuperSignal™ West Pico luminescence substrate kit (Thermo Fisher Scientific, Inc.) and incubated with the membranes. Protein bands were quantified using ImageJ v1.8.0 software (National Institutes of Health).

Statistical analysis

The statistical software packages SPSS 21.0 and GraphPad Prism 8.0 were used to analyze the experimental results. All data are presented as the mean ± standard deviations (means ± SD). The Kolmogorov-Smirnov test was used for each set of data and the data were found to be normally distributed. Differences between two groups were analyzed using unpaired t-tests. Differences in data from more than two groups were analyzed using one-way analysis of variance (ANOVA), followed by Tukey's multiple comparisons test. A value of P<0.05 was considered to indicate a statistically significant difference.

Results

Right ventricular remodeling and PVR in rats with PH

The echocardiogram of the blood flow in the pulmonary artery was detected using the Doppler ultrasonic diagnostic instrument. Compared with the CON group (Fig. 1A), the MCT group exhibited a midsystolic notch, with the peak shifting forward, and the PAAT value was decreased (P<0.01, n=6; Fig. 1B). The RVHI% of the rats in the MCT group was significantly higher than that in the CON group (P<0.01, n=6; Fig. 1C).

Figure 1

Right ventricular remodeling and pulmonary artery remodeling in pulmonary hypertension. (A) The pulmonary hemodynamic spectrum. (B) Comparison of PAAT. (C) Comparison of RVHI%. (D) H&E staining of the small pulmonary artery. (E) H&E staining of the middle pulmonary artery; scale bars, 50 µm (top panels) and 25 µm (bottom panels). The red arrows indicate the pulmonary artery. (F) Statistical analysis of the WT% and WA% of pulmonary arteries; scale bar, 50 or 25 µm. (G) Masson's trichrome staining of lung tissue. (H) Analysis of the fibrotic index of pulmonary vascular tissue; scale bar=50 or 25 µm. WT% (S), WT% of the small pulmonary artery; WA% (S), WA% of the small pulmonary artery; WT% (M), WT% of the middle pulmonary artery; WA% (m), WA% of the middle pulmonary artery. **P<0.01, MCT vs. CON (n=6, data were analyzed using a t-test). PAAT, pulmonary artery acceleration time; RVHI%, right ventricular hypertrophy index percentage; WT%, percentage of the thickness of the vessel wall; WA%, percentage of the vessel wall area; MCT, monocrotaline; CON, control.

H&E staining was performed to observe the structural differences between the small pulmonary arteries (diameter, 15-50 µm) (Fig. 1D) and the middle pulmonary arteries (diameter, 50-150 µm) (Fig. 1E). The pulmonary artery cells in the CON group were evenly distributed, continuous and structurally intact, while the pulmonary artery cells in the MCT group exhibited a disordered arrangement, the area of the lumen was reduced, and the thickness of the tube wall was significantly increased. The pulmonary artery WT% and WA% of the MCT group were significantly higher than that of the CON group (P<0.01, n=6; Fig. 1F).

Masson's trichrome staining was used to observe collagen deposition and lung fibrosis (Fig. 1G). The analysis revealed evident collagen deposition in the pulmonary blood vessels and lung tissues of the MCT group of rats (P<0.01, Fig. 1H). The lung tissue was evidently fibrotic.

Expression of KIR2.1, OPN, PCNA and TGF-β1/SMAD2/3 signaling pathway proteins in pulmonary blood vessels and lung tissues of rats with PH

Immunohistochemistry and immunofluorescence staining were performed to detect changes in the expression of KIR2.1 (Fig. 2A and B), the migration-related protein, OPN (Fig. 2A and C), and the proliferation-related protein, PCNA (Fig. 2A and D), in rat lung tissue. The KIR2.1, OPN and PCNA proteins were widely expressed in lung tissues and pulmonary blood vessels. The semi-quantitative analysis of the fluorescence intensity revealed that KIR2.1, OPN, and PCNA expression was significantly increased in the MCT group (P<0.01, n=6) compared with that in the CON group (Fig. 2E).

Figure 2

Expression of KIR2.1, OPN, PCNA and TGF-β1/SMAD2/3 signaling pathway proteins in pulmonary vessels and lung tissues of rats with pulmonary hypertension. (A) Immunohistochemical staining for KIR2.1, OPN and PCNA; scale bar, 25 µm. (B) Immunofluorescence staining for α-SMA and KIR2.1; (C) Immunofluorescence staining for α-SMA and OPN. (D) Immunofluorescence staining for α-SMA and PCNA. Scale bars, 50 µm. The white arrows indicate the pulmonary blood vessels. (E) Analysis of the relative expression of the KIR2.1, OPN and PCNA proteins using immunofluorescence staining. (F) Representative western blots illustrating KIR2.1, OPN, PCNA, TGF-β1, SMAD2, SMAD3, p-SMAD2, p-SMAD3 and GAPDH levels. (G) Analysis of the protein levels of KIR2.1, OPN, PCNA, TGF-β1, SMAD2, p-SMAD2, SMAD3 and p-SMAD3. *P<0.05 and **P<0.01, MCT vs. CON (n=6, data were analyzed using a t-test). KIR2.1, inwardly rectifying K+ channel 2.1; OPN, osteopontin; PCNA, proliferating cell nuclear antigen; α-SMA, α-smooth muscle actin; MCT, monocrotaline; CON, control.

Western blot analysis was conducted to further detect KIR2.1, OPN and PCNA protein levels in the tissue homogenate of rat pulmonary blood vessels (Fig. 2F). The results were consistent with those of the semi-quantitative analysis of immunofluorescence staining. Significantly higher levels of the KIR2.1, OPN and PCNA proteins were detected in the MCT group compared with the CON group (P<0.01 or P<0.05, n=6; Fig. 2G). Furthermore, western blot analysis was used to assess the expression of proteins in the TGF-β1/SMAD2/3 signaling pathway in tissue homogenates of pulmonary blood vessels (Fig. 2F). Following treatment with MCT, the levels of the TGF-β1 and p-SMAD2/3 proteins were significantly increased compared with those in the CON group (P<0.01 or P<0.05, n=6; Fig. 2G).

PDGF-BB upregulates KIR2.1 protein expression, and promotes the proliferation and migration of HPASMCs, which are inhibited by ML133

HPASMCs were treated with PDGF-BB and cell proliferation, migration and changes in KIR2.1 protein expression were observed to further investigate the role of KIR2.1 in PVR. Cell proliferation and migration were analyzed using scratch and Transwell assays (Fig. 3A and C). Following stimulation with PDGF-BB, the scratch healing ability of the HPASMCs was enhanced, and the number of cells that migrated to the lower surface of the Transwell™ chamber increased (P<0.01, n=6). Moreover, cell scratch healing and the number of cells migrating through the Transwell™ were reduced following pre-treatment with the KIR2.1 protein inhibitor, ML133 (P<0.01, n=6; Fig. 3B and D). The expression of the migration-related protein, OPN, and the proliferation-related protein, PCNA, in HPASMCs was detected using western blot analysis (Fig. 4A). The results were consistent with the phenotype. Following PDGF-BB intervention, the protein levels of OPN and PCNA in the HPASMCs were significantly increased (P<0.01, n=6), and OPN and the OPN and PCNA protein levels were significantly decreased in cells pre-treated with ML133 (P<0.01, n=6; Fig. 4C and D).

Figure 3

ML133 reverses the proliferation and migration of human pulmonary artery smooth muscle cells induced by PDGF-BB. (A) Cell scratch images; scale bar, 200 µm. (B) Crystal violet staining of the Transwell assay; scale bars, 100 µm (top panel) and 50 µm (bottom panel). (C) Quantitative analysis of the cell scratch healing rate. (D) Quantitative analysis of cell invasion. (E) Immunofluorescence staining for KIR2.1; scale bar, 50 µm. (F) Analysis of the relative expression of the KIR2.1 protein detected using immunofluorescence staining. **P<0.01, PDGF-BB vs. CON; ##P<0.01, PDGF-BB + ML133 vs. PDGF-BB (n=6, data were analyzed using one-way ANOVA). PDGF-BB, platelet-derived growth factor-BB; KIR2.1, inwardly rectifying K+ channel 2.1; CON, control.

Figure 4

ML133 alters the expression of related proteins in human pulmonary artery smooth muscle cells treated with PDGF-BB. (A) Representative western blots illustrating the levels of KIR2.1, OPN, PCNA, TGF-β1, SMAD2, SMAD3, p-SMAD2, p-SMAD3 and GAPDH. (B) Analysis of KIR2.1 expression. (C) Analysis of OPN expression. (D) Analysis of PCNA expression. (E) Analysis of TGF-β1 expression. (F) Analysis of p-SMAD2 and SMAD2 levels. (G) Analysis of p-SMAD3 and SMAD3 levels. *P<0.05 and **P<0.01, PDGF-BB vs. CON; #P<0.05 and ##P<0.01, PDGF-BB + ML133 vs. PDGF-BB (n=6, data were analyzed using one-way ANOVA). PDGF-BB, platelet-derived growth factor-BB; KIR2.1, inwardly rectifying K+ channel 2.1; OPN, osteopontin; PCNA, proliferating cell nuclear antigen; CON, control.

Immunofluorescence staining and western blot analysis were also performed to determine KIR2.1 protein expression in the HPASMCs (Figs. 3E and 4A). The results of immunofluorescence staining revealed that KIR2.1 was mainly located in the cell membrane and cytoplasm. The semi-quantitative analysis of the fluorescence intensity revealed a significantly higher protein expression of KIR2.1 in the HPASMCs stimulated with PDGF-BB than that in the CON group (P<0.01, n=6; Fig. 3E). However, KIR2.1 protein expression was decreased in the PDGF-BB + ML133 group (P<0.01, n=6; Fig. 3F). The results of western blot analysis were consistent with those from the semi-quantitative analysis of immunofluorescence staining. Following stimulation with PDGF-BB, KIR2.1 protein expression in the HPASMCs was significantly increased (P<0.01, n=6), whereas it was significantly decreased following pre-treatment with ML133 (P<0.01, n=6; Fig. 4B).

ML133 blocks the activation of the TGF-β1/SMAD2/3 signaling pathway induced by PDGF-BB

The levels of TGF-β1/SMAD2/3 signaling pathway proteins in HPASMCs treated with PDGF-BB were examined using western blot analysis to investigate the underlying molecular mechanisms (Fig. 4A). PDGF-BB increased the levels of TGF-β1, p-SMAD2 and p-SMAD3 in HPASMCs (P<0.01 or P<0.05, n=6) and activated the TGF-β1/SMAD2/3 signaling pathway. Following pre-treatment with ML133, the levels of TGF-β1 and p-SMAD2/3 were significantly decreased (P<0.01 or P<0.05, n=6), and the TGF-β1/SMAD2/3 signaling pathway was inhibited (Fig. 4E-G).

The TGF-β1/SMAD2/3 blocker, SB431542, inhibits the proliferation and migration of HPASMCs, but does not affect KIR2.1 expression

The HPASMCs were pre-treated with the TGF-β1/SMAD2/3 inhibitor, SB431542, and the changes in cell proliferation were observed following the PDGF-BB intervention to further elucidate the role of the TGF-β1/SMAD2/3 signaling pathway in HPASMC proliferation and migration. Compared with the PDGF-BB group, the cell scratch healing level was reduced in the PDGF-BB + SB431542 group (Fig. 5A and C), and the number of cells migrating through the Transwell™ was decreased (P<0.01 or P<0.05, n=6; Fig. 5B and D), indicating that cell proliferation and migration were decreased. The expression of the OPN and PCNA proteins was examined using western blot analysis (Fig. 6A), and the results were consistent with the phenotype. Following pre-treatment with SB431542, OPN and PCNA protein expression was significantly decreased (P<0.01 or P<0.05, n=6; Fig. 6C and D).

Figure 5

SB431542 reverses the proliferation and migration of human pulmonary artery smooth muscle cells induced by PDGF-BB. (A) Cell scratch images; scale bar, 200 µm. (B) Crystal violet staining results from the Transwell assay; scale bars, 100 µm (top panel) and 50 µm (bottom panel). (C) Quantitative analysis of the cell scratch healing rate. (D) Quantitative analysis of cell invasion. (E) Immunofluorescence staining for KIR2.1; scale bar, 50 µm. (F) Analysis of the relative expression of the KIR2.1 protein using immunofluorescence staining. **P<0.01, PDGF-BB vs. CON; #P<0.05 and ##P<0.01, PDGF-BB + SB431542 vs. PDGF-BB (n=6, data were analyzed using one-way ANOVA). PDGF-BB, platelet-derived growth factor-BB; KIR2.1, inwardly rectifying K+ channel 2.1; CON, control. PDGF-BB, platelet-derived growth factor-BB; KIR2.1, inwardly rectifying K+ channel 2.1; CON, control.

Figure 6

SB431542 alters the expression of related proteins in human pulmonary artery smooth muscle cells treated with PDGF-BB. (A) Representative western blots illustrating the levels of KIR2.1, OPN, PCNA, TGF-β1, SMAD2, SMAD3, p-SMAD2, p-SMAD3 and GAPDH. (B) Analysis of KIR2.1 levels. (C) Analysis of OPN levels. (D) Analysis of PCNA levels. (E) Analysis of p-SMAD2 and SMAD2 levels. (F) Analysis of p-SMAD3 and SMAD3 levels. **P<0.01, PDGF-BB vs. CON; #P<0.05 and ##P<0.01, PDGF-BB + SB431542 vs. PDGF-BB (n=6, data were analyzed using one-way ANOVA). PDGF-BB, platelet-derived growth factor-BB; KIR2.1, inwardly rectifying K+ channel 2.1; OPN, osteopontin; PCNA, proliferating cell nuclear antigen; CON, control.

Based on the aforementioned results, it was found that PDGF-BB upregulates KIR2.1 protein expression in HPASMCs and activates the TGF-β1/SMAD2/3 signaling pathway. The present study then further investigated the upstream and downstream association between KIR2.1 and the TGF-β1/SMAD2/3 signaling pathway by detecting changes in KIR2.1 levels following pre-treatment with SB431542. The results of immunofluorescence staining and western blot analysis (Figs. 5E and 6A) revealed that the SMAD2/3 signaling pathway was inhibited following pre-treatment with SB431542 (P<0.01 or P<0.05, n=6; Fig. 6E and F); however, no significant differences in KIR2.1 protein expression were observed between the PDGF-BB + SB431542 group and the PDGF-BB group (P>0.05, n=6; Figs. 5F and 6B).

On the whole, PDGF-BB activated the TGF-β1/SMAD2/3 signaling pathway and upregulated the expression of OPN and PCNA to promote cell proliferation and migration. ML133 inhibited KIR2.1 channel activation and modulated the downstream TGF-β1/SMAD2/3 signaling pathway. The activation of the TGF-β1/SMAD2/3 signaling pathway was blocked by SB431542; however, the expression of KIR2.1 was not affected, indicating that KIR2.1 is located upstream of the TGF-β1/SMAD2/3 signaling pathway (Fig. 7).

Figure 7

KIR2.1 is involved in PDGF-BB-induced cell proliferation and migration. PDGF-BB activated the TGF-β1/SMAD2/3 signaling pathway and upregulated the expression of OPN and PCNA to promote cell proliferation and migration. ML133 inhibited KIR2.1 channel activation and modulated the downstream TGF-β1/SMAD2/3 signaling pathway. The activation of the TGF-β1/SMAD2/3 signaling pathway was blocked by SB431542; however, the expression of KIR2.1 was not affected, indicating that KIR2.1 is located upstream of the TGF-β1/SMAD2/3 signaling pathway. PDGF-BB upregulated KIR2.1 protein expression, activated the KIR2.1 channel, and promoted K+ ion influx (red arrow) and cell membrane depolarization. Subsequently, the TGF-β1/SMAD2/3 signaling pathway was activated to regulate the proliferation and migration of pulmonary artery smooth muscle cells. KIR2.1, inwardly rectifying K+ channel 2.1; PDGF-BB, platelet-derived growth factor-BB; OPN, osteopontin; PCNA, proliferating cell nuclear antigen.

Discussion

The main findings of the present study were the following: Significant right ventricular remodeling and PVR were observed in the rats with PH, and obvious PVR and pulmonary fibrosis were detected. The expression of the KIR2.1, OPN and PCNA proteins in pulmonary vessels and lung tissues increased, and the TGF-β1/SMAD2/3 signaling pathway was activated. PDGF-BB upregulated the expression of the KIR2.1 protein, activated the TGF-β1/SMAD2/3 signaling pathway, and promoted the proliferation and migration of smooth muscle cells. The KIR2.1 protein inhibitor, ML133, blocked the activation of the TGF-β1/SMAD2/3 signaling pathway by PDGF-BB, and inhibited the proliferation and migration of smooth muscle cells. SB431542, an inhibitor of TGF-β1/SMAD2/3 signaling, reduced the proliferation and migration of HPASMCs induced by PDGF-BB, but did not affect KIR2.1 expression.

The main characteristics of PH are PVR and right ventricular hypertrophy (1). An intraperitoneal injection of MCT is one of the most classic methods used to establish PH models. In the present study, the MCT group exhibited a decrease in PAAT and an increase in RVHI%. In addition, the expression of the migration-related protein, OPN, and the proliferation-related protein, PCNA, was detected in pulmonary blood vessels and lung tissues. The results revealed significantly higher levels of the OPN and PCNA proteins in rats with PH than in the control group. The regulatory role of PDGF-BB in VSMC proliferation, migration and phenotype has been extensively studied (40,41). In the present study, in vitro, it was found that PDGF-BB stimulation promoted HPASMC proliferation and migration, and upregulated OPN and PCNA protein expression in the cells.

K+ channels are closely related to the proliferation and apoptosis of PASMCs (42). To date, four different types of K+ channels have been identified in smooth muscle cells: Voltage-gated K+ (Kv) channels (43,44), ATP-sensitive potassium (KATP) channels (45), large conductance Ca2+ -activated K+ (BK Ca) channels (44,46,47) and KIR channels (22,41,48). The contribution of KIR2.1 to proliferation and migration is relatively controversial and may also be dependent on the cell type. In human heart c-kit+ progenitor cells, the cell membrane potential is depolarized by silencing the KIR2.1 channel, and cell proliferation is not affected, although the cell migration rate is increased (16). In microglia, the inhibition of KIR2.1 has been found to increase cell proliferation, but to reduce cell migration (17). In rat VSMC, the knockdown of KIR2.1 expression has been shown to inhibit PDGF-BB-induced cell proliferation, migration, phenotype and intimal hyperplasia following balloon injury (22). In the human thoracic aorta, KIR2.1 has been found to be related to VSMC proliferation and vascular remodeling (18). In the present study, the protein expression of KIR2.1 was detected in pulmonary blood vessels and lung tissues, and a significantly higher protein expression of KIR2.1 was observed in pulmonary arteries and lung tissues of rats with PH than in the control group. KIR2.1 is closely related to PH development and PVR. In vitro, PDGF-BB promoted cell proliferation and migration, while upregulating the protein expression of KIR2.1 in cells. Following treatment with the KIR2.1 inhibitor, ML133, the proliferation and migration induced by PDGF-BB were inhibited, while the protein expression levels of OPN and PCNA were decreased. Therefore, the present study demonstrated that the KIR2.1 channel participates in PASMC proliferation and migration, as well as in PVR. The present study aimed to verify the current changes in KIR2.1 function using the whole-cell patch clamp technique. However, due to the limited HPASMC volume and small KIR2.1 current, the experimental process and results were not ideal. Therefore, the whole-cell patch clamp technique could not be used for validation experiments in the present study.

For cell proliferation, cell viability (49), apoptosis [expression of apoptosis-related genes (Bcl-2, cleaved caspase-3 and Bax)] (50) and the cell cycle (51) can further influence cell proliferation. The inhibition of KIR2.1 channel-induced depolarization has been shown to promote the biological activity and differentiation of late endothelial progenitor cells (52). Another study found that the inhibition of KIR2.1 channels increased cell mobility without affecting cell cycling progression in human cardiac c-kit+ progenitor cells (16). A previous study on BV2 microglial cells demonstrated that hypoxia induced the apoptosis of cells by upregulating KIR2.1 protein to activate mitochondria-related apoptotic pathways (50). However, such experiments have not been previously reported using PASMCs, at least to the best of our knowledge. Therefore, whether KIR2.1 channels can affect the proliferation and migration of PASMCs by regulating cell proliferation, cell viability and apoptosis warrants further investigation.

The TGF-β signaling pathway regulates various cardiovascular diseases (53). In the MCT-induced PH model, the expression of the TGF-β1 protein and the levels of p-SMAD2/3 have been shown to be significantly increased, promoting the proliferation and migration of smooth muscle cells, PVR and increasing pulmonary vascular resistance, ultimately accelerating the occurrence and development of PH (54). In the present study, in vivo, the levels of TGF-β1 and p-SMAD2/3 proteins in rats with PH were higher than those in the control group, and the TGF-β1/SMAD2/3 signaling pathway was activated, consistent with the findings of previous research (54). In vitro, PDGF-BB increased the TGF-β1 and p-SMAD2/3 protein levels in HPASMCs, and activated the TGF-β1/SMAD2/3 signaling pathway. At the same time, TGF-β1 is also an indicator of fibrosis (55). In vivo, the present study found that the pulmonary blood vessels and lung tissues of the rats with PH induced by MCT exhibited obvious fibrosis. TGF-β1 is closely related to a depolarized membrane potential (56). When applied for 24 to 48 h, TGF-β1 causes substantial membrane depolarization concomitant with a several-fold increase of transmembrane currents (56). In the in vitro experiments in the present study, following the inhibition of KIR2.1, the protein expression levels of TGF-β1 and p-SMAD2/3 were decreased, and the TGF-β1/SMAD2/3 signaling pathway was inhibited. The activation of the KIR2.1 channel promotes K+ ion influx and cell membrane depolarization and may regulate the TGF-β1/SMAD2/3 signaling pathway to alter the proliferation and migration of HPASMCs.

In the present study, he TGF-β1/SMAD2/3 signaling pathway inhibitor, SB431542, was used to further verify the upstream and downstream relationship between KIR2.1 and the TGF-β1/SMAD2/3 signaling pathway. The blockade of the TGF-β1/SMAD2/3 signaling pathway reversed PDGF-BB-induced cell proliferation and migration, and reduced the OPN and PCNA protein levels. However, blocking the TGF-β1/SMAD2/3 signaling pathway did not affect the protein expression of KIR2.1. Therefore, KIR2.1 is located upstream of the TGF-β1/SMAD2/3 signaling pathway. PDGF-BB upregulates KIR2.1 protein expression, activates the KIR2.1 channel, promotes K+ ion influx and cell membrane depolarization, and then activates the TGF-β1/SMAD2/3 signaling pathway to regulate PASMC proliferation and migration (Fig. 7).

In conclusion, the present study demonstrates that KIR2.1 is involved in PVR and the generation of PH, potentially as the KIR2.1 channel regulates the activity of the TGF-β1/SMAD2/3 signaling pathway by regulating cell membrane depolarization, thereby modulating the proliferation and migration of PASMCs and participating in PVR. Therefore, KIR2.1 may serve as a potential therapeutic target for the prevention or treatment of PH.