Yaya Kassogue1,2,3, Brehima Diakite1,2,3, Mamoudou Maiga2,3,4, Oumar Kassogue2, Issa Konate3,5, Kadidiatou Tamboura5, Fousseyni Diarra2, Zoumana Diarra6, Mahamadou Karamoko Sawadogo6, Yaya Goita7,8, Sidi Boula Sissoko3,9, Adama Seydou Sissoko3,10, Nouhoum Guirou3,11, Hind Dehbi12,13, Sellama Nadifi12,13, Sekou Bah8,14, Cheick Bougadari Traore1,2,3, Bakarou Kamate1,2,3, Sounkalo Dao3,5, Guimogo Dolo2,3. 1. Department of Anatomo-pathology, University Hospital of Point G. 2. Laboratory of Research and training on Molecular Pathologies, University Hospital of Point G. 3. Faculty of Medicine and Odontostomatology, University of Sciences, Techniques and Technologies of Bamako. 4. Department of Biomedical Engineering, Northwestern University, Evanston, Illinois, USA. 5. Department of Infectious Diseases and Tropical Medicine, University Hospital of Point G. 6. Center of Listening, Care, Animation, and Counseling for People Living With HIV. 7. Department of Medical Biology and Anatomo-pathology, University Hospital, Mali Hospital. 8. Faculty of Pharmacy, University of Sciences, Techniques and Technologies of Bamako. 9. Department of Cytogenetics and Reproductive Biology, National Institute for Public Health Research. 10. Department of Neurology, University Hospital of Point G. 11. Institute of Tropical Ophthalmology of Africa, Bamako, Mali. 12. Cellular and Molecular Pathology Laboratory, Faculty of Medicine and Pharmacy of Casablanca, University Hassan II. 13. Medical Genetics Laboratory, Ibn Rochd University Hospital Center, Casablanca, Morocco. 14. Department of pharmacy, University Hospital of Point G, Bamako, Mali.
Abstract
OBJECTIVES: The main objective of this study was to evaluate the effect of CYP2B6 and CYP3A4 polymorphisms on the virological and immunologic responses of HIV patients. A total of 153 HIV-positive patients were enlisted for the study. PATIENTS AND METHODS: Viral load and median CD4 T cell counts were evaluated at baseline and month 6 (M6). Samples were identified using TaqMan genotyping assays. RESULTS: The AG in CYP2B6 rs2279343 was associated with VLS compared to homozygous AA. In the dominant model, the AG/GG genotypes were associated with VLS compared to the AA genotype. Moreover, in overdominant model, the AG genotype was associated with VLS compared to AA/GG. Regarding immunological response, only the AG in SNP rs2279343 CYP2B6 was associated with an increase in CD4 cell count between baseline and M6. In CYP2B6 rs3745274, the CD4 cell count at M6 was higher than that of baseline for GG carriers and for GT carriers. In CYP3A4 rs2740574, the TC carriers showed a higher median CD4 count at M6 compared to that of the baseline count, as well as for CC carriers. The best genotypes combination associated with CD4 cell count improvement were AA/AG in SNP rs2279343 and GG/GT in SNP rs3745274. CONCLUSION: Our findings support the fact that CYP2B6 rs2279343 could help in the prediction of VLS and both SNPs rs3745274 and rs2279343 in CYP2B6 and CYP3A4 rs2740574 were associated with immune recovery in Malian HIV-positive patients.
OBJECTIVES: The main objective of this study was to evaluate the effect of CYP2B6 and CYP3A4 polymorphisms on the virological and immunologic responses of HIV patients. A total of 153 HIV-positive patients were enlisted for the study. PATIENTS AND METHODS: Viral load and median CD4 T cell counts were evaluated at baseline and month 6 (M6). Samples were identified using TaqMan genotyping assays. RESULTS: The AG in CYP2B6 rs2279343 was associated with VLS compared to homozygous AA. In the dominant model, the AG/GG genotypes were associated with VLS compared to the AA genotype. Moreover, in overdominant model, the AG genotype was associated with VLS compared to AA/GG. Regarding immunological response, only the AG in SNP rs2279343 CYP2B6 was associated with an increase in CD4 cell count between baseline and M6. In CYP2B6 rs3745274, the CD4 cell count at M6 was higher than that of baseline for GG carriers and for GT carriers. In CYP3A4 rs2740574, the TC carriers showed a higher median CD4 count at M6 compared to that of the baseline count, as well as for CC carriers. The best genotypes combination associated with CD4 cell count improvement were AA/AG in SNP rs2279343 and GG/GT in SNP rs3745274. CONCLUSION: Our findings support the fact that CYP2B6 rs2279343 could help in the prediction of VLS and both SNPs rs3745274 and rs2279343 in CYP2B6 and CYP3A4 rs2740574 were associated with immune recovery in Malian HIV-positive patients.
Authors: Anne F Luetkemeyer; Susan L Rosenkranz; Darlene Lu; Beatriz Grinsztejn; Jorge Sanchez; Michael Ssemmanda; Ian Sanne; Helen McIlleron; Diane V Havlir; David W Haas Journal: Clin Infect Dis Date: 2015-02-26 Impact factor: 9.079
Authors: Sandi L Navarro; Misty R Saracino; Karen W Makar; Sushma S Thomas; Lin Li; Yingye Zheng; Lisa Levy; Yvonne Schwarz; Jeannette Bigler; John D Potter; Johanna W Lampe Journal: J Nutrigenet Nutrigenomics Date: 2011-05-28
Authors: M Rotger; H Tegude; S Colombo; M Cavassini; H Furrer; L Décosterd; J Blievernicht; T Saussele; H F Günthard; M Schwab; M Eichelbaum; A Telenti; U M Zanger Journal: Clin Pharmacol Ther Date: 2007-01-18 Impact factor: 6.875
Authors: Sandra G Heil; Marchina E van der Ende; Paul W Schenk; Ilse van der Heiden; Jan Lindemans; David Burger; Ron H N van Schaik Journal: Ther Drug Monit Date: 2012-04 Impact factor: 3.681
Authors: Zeruesenay Desta; Ahmed El-Boraie; Li Gong; Andrew A Somogyi; Volker M Lauschke; Collet Dandara; Kathrin Klein; Neil A Miller; Teri E Klein; Rachel F Tyndale; Michelle Whirl-Carrillo; Andrea Gaedigk Journal: Clin Pharmacol Ther Date: 2021-03-11 Impact factor: 6.903