Literature DB >> 3585251

Cholesterol requirement of P3-X63-Ag8 and X63-Ag8.653 mouse myeloma cells for growth in vitro.

J D Sato, T Kawamoto, T Okamoto.   

Abstract

P3-X63-Ag8 and X63-Ag8.653 mouse myeloma cells have an absolute requirement for cholesterol for growth under serum-free conditions. This requirement can be satisfied by low density lipoprotein at 2-6 micrograms/ml or by BSA-bound cholesterol at 5-10 micrograms/ml. Cholesterol-independent variants can be selected after prolonged growth in low concentrations of serum.

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Year:  1987        PMID: 3585251      PMCID: PMC2188360          DOI: 10.1084/jem.165.6.1761

Source DB:  PubMed          Journal:  J Exp Med        ISSN: 0022-1007            Impact factor:   14.307


  19 in total

1.  Continuous cultures of fused cells secreting antibody of predefined specificity.

Authors:  G Köhler; C Milstein
Journal:  Nature       Date:  1975-08-07       Impact factor: 49.962

Review 2.  The low-density lipoprotein pathway and its relation to atherosclerosis.

Authors:  J L Goldstein; M S Brown
Journal:  Annu Rev Biochem       Date:  1977       Impact factor: 23.643

Review 3.  Antibiotic interaction with model membranes.

Authors:  S C Kinsky
Journal:  Annu Rev Pharmacol       Date:  1970       Impact factor: 13.820

4.  Human lymphoblastoid lines from lymph node and spleen.

Authors:  J A Levy; M Virolainen; V Defendi
Journal:  Cancer       Date:  1968-09       Impact factor: 6.860

5.  Mouse myelomas and lymphomas in culture.

Authors:  K Horibata; A W Harris
Journal:  Exp Cell Res       Date:  1970-04       Impact factor: 3.905

6.  Fusion between immunoglobulin-secreting and nonsecreting myeloma cell lines.

Authors:  G Köhler; S C Howe; C Milstein
Journal:  Eur J Immunol       Date:  1976-04       Impact factor: 5.532

7.  Establishment and characterization of a human histiocytic lymphoma cell line (U-937).

Authors:  C Sundström; K Nilsson
Journal:  Int J Cancer       Date:  1976-05-15       Impact factor: 7.396

8.  Animal cell mutants defective in sterol metabolism: a specific selection procedure and partial characterization of defects.

Authors:  Y Saito; S M Chou; D F Silbert
Journal:  Proc Natl Acad Sci U S A       Date:  1977-09       Impact factor: 11.205

9.  Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes.

Authors:  N N Iscove; F Melchers
Journal:  J Exp Med       Date:  1978-03-01       Impact factor: 14.307

10.  Regulation of cholesterol synthesis by low density lipoprotein in isolated human lymphocytes. Comparison of cells from normal subjects and patients with homozygous familial hypercholesterolemia and abetalipoproteinemia.

Authors:  Y K Ho; J R Faust; D W Bilheimer; M S Brown; J L Goldstein
Journal:  J Exp Med       Date:  1977-06-01       Impact factor: 14.307

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  29 in total

1.  Adaptation of cholesterol-requiring NS0 mouse myeloma cells to high density growth in a fully defined protein-free and cholesterol-free culture medium.

Authors:  M J Keen; T W Steward
Journal:  Cytotechnology       Date:  1995-10       Impact factor: 2.058

2.  Functional expression of human and mouse low density lipoprotein receptors in hybridomas.

Authors:  Y Shintani; K Iwamoto; K Kitano
Journal:  Cytotechnology       Date:  1995-01       Impact factor: 2.058

3.  Eldecalcitol (ED-71), an analog of 1α,25(OH)2D3, inhibits the growth of squamous cell carcinoma (SCC) cells in vitro and in vivo by down-regulating expression of heparin-binding protein 17/fibroblast growth factor-binding protein-1 (HBp17/FGFBP-1) and FGF-2.

Authors:  T Shintani; F Takatsu; S N Z Rosli; E Usui; A Hamada; K Sumi; Y Hayashido; S Toratani; Tetsuji Okamoto
Journal:  In Vitro Cell Dev Biol Anim       Date:  2017-07-14       Impact factor: 2.416

4.  Expression of fibroblast growth factor binding protein HBp17 in normal and tumor cells.

Authors:  T Okamoto; Y Tanaka; M Kan; A Sakamoto; K Takada; J D Sato
Journal:  In Vitro Cell Dev Biol Anim       Date:  1996-02       Impact factor: 2.416

5.  An alternative method for the isolation of NS-1 hybridomas using cholesterol auxotrophy of NS-1 mouse myeloma cells.

Authors:  Y Myoken; T Okamoto; T Osaki; M Yabumoto; G H Sato; K Takada; J D Sato
Journal:  In Vitro Cell Dev Biol       Date:  1989-05

6.  Leukemia inhibitory factor as an anti-apoptotic mitogen for pluripotent mouse embryonic stem cells in a serum-free medium without feeder cells.

Authors:  Miho Furue; Tetsuji Okamoto; Yohei Hayashi; Hitoshi Okochi; Manabu Fujimoto; Yasufumi Myoishi; Takanori Abe; Kiyoshi Ohnuma; Gordon H Sato; Makoto Asashima; J Denry Sato
Journal:  In Vitro Cell Dev Biol Anim       Date:  2005 Jan-Feb       Impact factor: 2.416

7.  Establishment of a macrophagelike cell line derived from U-937, human histiocytic lymphoma, grown serum-free.

Authors:  Z L Kong; M Miwa; H Murakami; K Shinohara
Journal:  In Vitro Cell Dev Biol       Date:  1990-10

8.  1α,25(OH)₂D₃ inhibits FGF-2 release from oral squamous cell carcinoma cells through down-regulation of HBp17/FGFBP-1.

Authors:  S N Zawani B Rosli; Tomoaki Shintani; Shigeaki Toratani; Emiko Usui; Tetsuji Okamoto
Journal:  In Vitro Cell Dev Biol Anim       Date:  2014-06-18       Impact factor: 2.416

9.  Advantages and difficulties in culturing human pluripotent stem cells in growth factor-defined serum-free medium.

Authors:  Miho Kusuda Furue; Daiki Tateyama; Masaki Kinehara; Jie Na; Tetsuji Okamoto; J Denry Sato
Journal:  In Vitro Cell Dev Biol Anim       Date:  2010-04-29       Impact factor: 2.416

10.  Syntheses of prostaglandin E2 and E-cadherin and gene expression of beta-defensin-2 by human gingival epithelial cells in response to Actinobacillus actinomycetemcomitans.

Authors:  Takuji Noguchi; Hideki Shiba; Hitoshi Komatsuzawa; Noriyoshi Mizuno; Yuushi Uchida; Kazuhisa Ouhara; Ryuji Asakawa; Seiji Kudo; Hiroyuki Kawaguchi; Motoyuki Sugai; Hidemi Kurihara
Journal:  Inflammation       Date:  2003-12       Impact factor: 4.092

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