Ke Chen1, Shaohua Wang2. 1. Department of Gastroenterology, Hubei Third People's Hospital Affiliated to Jianghan University, Wuhan, Hubei 430030, China. 2. Emergency Department, Hubei Third People's Hospital Affiliated to Jianghan University, Wuhan, Hubei 430030, China.
Abstract
Objective: The lack of certain trace elements such as selenium, molybdenum, magnesium or related nutrients in the soil, water quality and food in the disease area, which caused disturbance of myocardium metabolism and resulted in injury and necrosis. The aim of the study was to explore the mechanism of ibuprofen alleviating myocardial injury caused by acute pancreatitis (AP). Method: We have established AP cell model and rat model. HE staining is used for histological examination. ELISA is used to determine the levels of proinflammatory cytokines (TNF-α and IL-6) and markers of myocardial injury (LDH and CK-MB). qRT-PCR and Western blot are used to analyze the mRNA and protein levels of related genes. Results: The expression level of AIM2 was significantly increased in AP cells; downregulation of AIM2 alleviated inflammation and myocardial injury induced by AP cells; ibuprofen could inhibit the expression of AIM2 and alleviate inflammation and myocardial injury induced by AP cells. In vivo experiments have found that ibuprofen can inhibit the expression of AIM2 to alleviate myocardial injury in AP rat. Conclusion: Ibuprofen can alleviate myocardial injury caused by acute pancreatitis by inhibiting the expression of AIM2.
Objective: The lack of certain trace elements such as selenium, molybdenum, magnesium or related nutrients in the soil, water quality and food in the disease area, which caused disturbance of myocardium metabolism and resulted in injury and necrosis. The aim of the study was to explore the mechanism of ibuprofen alleviating myocardial injury caused by acute pancreatitis (AP). Method: We have established AP cell model and rat model. HE staining is used for histological examination. ELISA is used to determine the levels of proinflammatory cytokines (TNF-α and IL-6) and markers of myocardial injury (LDH and CK-MB). qRT-PCR and Western blot are used to analyze the mRNA and protein levels of related genes. Results: The expression level of AIM2 was significantly increased in AP cells; downregulation of AIM2 alleviated inflammation and myocardial injury induced by AP cells; ibuprofen could inhibit the expression of AIM2 and alleviate inflammation and myocardial injury induced by AP cells. In vivo experiments have found that ibuprofen can inhibit the expression of AIM2 to alleviate myocardial injury in AP rat. Conclusion: Ibuprofen can alleviate myocardial injury caused by acute pancreatitis by inhibiting the expression of AIM2.
Authors: Marcus Hollenbach; Sebastian Sonnenberg; Ines Sommerer; Jana Lorenz; Albrecht Hoffmeister Journal: PLoS One Date: 2021-01-25 Impact factor: 3.240