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Literature DB >> 3584077

Molecular cloning and biological characterization of the recA gene from Pseudomonas syringae.

M J Hickman, C S Orser, D K Willis, S E Lindow, N J Panopoulos.

Abstract

We have identified a recombinant plasmid, pCUV8, from a cosmid library of Pseudomonas syringae genomic DNA which contains a functional analog of the Escherichia coli recA gene. The plasmid was initially identified by its ability to restore UV resistance to E. coli HB101. Quantitative analysis demonstrated that it restored both recombination proficiency and UV resistance to an E. coli recA deletion mutant. By these criteria, pCUV8 appears to contain the P. syringae recA gene. Several pathogenic and epiphytic strains of P. syringae, but not E. coli, showed sequence homology to pCUV8 under normal stringency.

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Year: 1987 PMID： 3584077 PMCID： PMC212212 DOI： 10.1128/jb.169.6.2906-2910.1987

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

24 in total

1. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.

Authors: G M Wahl; M Stern; G R Stark
Journal: Proc Natl Acad Sci U S A Date: 1979-08 Impact factor: 11.205

6. Multiple loci of Pseudomonas syringae pv. syringae are involved in pathogenicity on bean: restoration of one lesion-deficient mutant requires two tRNA genes.

Authors: J J Rich; D K Willis
Journal: J Bacteriol Date: 1997-04 Impact factor: 3.490

Molecular cloning and biological characterization of the recA gene from Pseudomonas syringae.

1. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.

2. Simple method for identification of plasmid-coded proteins.

Review 3. Pedigrees of some mutant strains of Escherichia coli K-12.

4. In vivo studies of temperature-sensitive recB and recC mutants.

5. Genetic location of certain mutations conferring recombination deficiency in Escherichia coli.

6. Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12.

7. A complementation analysis of the restriction and modification of DNA in Escherichia coli.

Review 8. Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli.

Review 9. Recombination deficient mutants of E. coli and other bacteria.

10. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans.

1. Cloning of the recA gene from a free-living leptospire and distribution of RecA-like protein among spirochetes.

2. Cloning and characterization of the Haemophilus influenzae Rd rec-1+ gene.

3. Construction of an Agrobacterium tumefaciens C58 recA mutant.

4. Characterization of the rec-1 gene of Haemophilus influenzae and behavior of the gene in Escherichia coli.

5. Pathovar-specific requirement for the Pseudomonas syringae lemA gene in disease lesion formation.

6. Multiple loci of Pseudomonas syringae pv. syringae are involved in pathogenicity on bean: restoration of one lesion-deficient mutant requires two tRNA genes.

7. recA gene of Escherichia coli complements defects in DNA repair and mutagenesis in Streptomyces fradiae JS6 (mcr-6).

8. Characterization of recA genes and recA mutants of Rhizobium meliloti and Rhizobium leguminosarum biovar viciae.

9. Transcriptional and translational analyses of recA mutant alleles in Pseudomonas aeruginosa.