Literature DB >> 3583983

The effects of various nutritional supplements on the growth, migration and differentiation of Xenopus laevis neural crest cells in vitro.

H C Wilson, N C Milos.   

Abstract

This study investigates the nutritional requirements of Xenopus laevis neural crest cells and melanophores developing in vitro. A comparison is made between the growth and differentiation of cells in serum-containing medium and a chemically defined, serum-free medium that we have designed. Our chemically defined medium is more efficient than serum-supplemented medium in promoting proliferation of these cells. Several supplements are required to enhance culture development. These include insulin, alpha-melanocyte stimulating hormone, somatotropin, luteotrophic hormone, linoleic acid, uridine, and putrescine. In addition, collagen and fibronectin provide the most conducive environment tested for cell migration and adhesion.

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Year:  1987        PMID: 3583983     DOI: 10.1007/bf02620988

Source DB:  PubMed          Journal:  In Vitro Cell Dev Biol        ISSN: 0883-8364


  47 in total

1.  Observations on nudging cells in culture.

Authors:  C Tickle; J P Trinkaus
Journal:  Nature       Date:  1976-06-03       Impact factor: 49.962

2.  The growth of mouse melanoma cells in hormone-supplemented, serum-free medium.

Authors:  J P Mather; G H Sato
Journal:  Exp Cell Res       Date:  1979-04       Impact factor: 3.905

3.  Basement membrane glycoprotein laminin binds to heparin.

Authors:  S Sakashita; E Engvall; E Ruoslahti
Journal:  FEBS Lett       Date:  1980-07-28       Impact factor: 4.124

4.  Long-term cultivation of amphibian melanophores. In vitro ageing and spontaneous transformation to a continuous cell line.

Authors:  H Kondo; H Ide
Journal:  Exp Cell Res       Date:  1983-11       Impact factor: 3.905

5.  Role of collagen and fibronectin in neural crest cell adhesion and migration.

Authors:  J H Greenberg; S Seppä; H Seppä; A Tyl Hewitt
Journal:  Dev Biol       Date:  1981-10-30       Impact factor: 3.582

6.  Cellular fibronectin promotes adrenergic differentiation of quail neural crest cells in vitro.

Authors:  M Sieber-Blum; F Sieber; K M Yamada
Journal:  Exp Cell Res       Date:  1981-06       Impact factor: 3.905

7.  Ceruloplasmin, a copper-transport protein, can act as a growth promoter for some cell lines in serum-free medium.

Authors:  J P Mather
Journal:  In Vitro       Date:  1982-12

8.  Differential modulation by spermidine of reactions catalyzed by type 1 prokaryotic and eukaryotic topoisomerases.

Authors:  K S Srivenugopal; D R Morris
Journal:  Biochemistry       Date:  1985-08-27       Impact factor: 3.162

9.  Selenium is an essential trace nutrient for growth of WI-38 diploid human fibroblasts.

Authors:  W L McKeehan; W G Hamilton; R G Ham
Journal:  Proc Natl Acad Sci U S A       Date:  1976-06       Impact factor: 11.205

10.  Effects of vitamin A on the behaviour of migratory neural crest cells in vitro.

Authors:  P Thorogood; L Smith; A Nicol; R McGinty; D Garrod
Journal:  J Cell Sci       Date:  1982-10       Impact factor: 5.285

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  2 in total

1.  Modulation of potassium channel function confers a hyperproliferative invasive phenotype on embryonic stem cells.

Authors:  Junji Morokuma; Douglas Blackiston; Dany S Adams; Guiscard Seebohm; Barry Trimmer; Michael Levin
Journal:  Proc Natl Acad Sci U S A       Date:  2008-10-17       Impact factor: 11.205

2.  A serum-free primary culture system for studying cell-substrate interactions during newt epidermal cell migration.

Authors:  J T Mahan; D J Donaldson
Journal:  In Vitro Cell Dev Biol       Date:  1988-10
  2 in total

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