Literature DB >> 35838971

Measurement of Mitochondrial Respiration in Cryopreserved Human Peripheral Blood Mononuclear Cells (PBMCs).

Keiko Iwata1,2,3, Min-Jue Xie4,5,6, Paul C Guest7, Takaharu Hirai8, Hideo Matsuzazki4,5,6.   

Abstract

Inflammatory diseases caused by infectious agents such as the SARS-CoV-2 virus can lead to impaired reductive-oxidative (REDOX) balance and disrupted mitochondrial function. Peripheral blood mononuclear cells (PBMCs) provide a useful model for studying the effects of inflammatory diseases on mitochondrial function but can be limited by the need to store these cells by cryopreservation prior to assay. Here, we describe a method for improving and determining PBMC viability with normalization of values to number of living cells. The approach can be applied not only to PBMC samples derived from patients with diseases marked by an altered inflammatory response such as viral infections.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  COVID-19; Cryopreservation; Inflammatory disease; Mitochondrial respiration; PBMCs; SARS-CoV-2

Mesh：

Year:  2022        PMID： 35838971     DOI： 10.1007/978-1-0716-2395-4_24

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  11 in total

1.  Elevated Glucose Levels Favor SARS-CoV-2 Infection and Monocyte Response through a HIF-1α/Glycolysis-Dependent Axis.

Authors:  Ana Campos Codo; Gustavo Gastão Davanzo; Lauar de Brito Monteiro; Gabriela Fabiano de Souza; Stéfanie Primon Muraro; João Victor Virgilio-da-Silva; Juliana Silveira Prodonoff; Victor Corasolla Carregari; Carlos Alberto Oliveira de Biagi Junior; Fernanda Crunfli; Jeffersson Leandro Jimenez Restrepo; Pedro Henrique Vendramini; Guilherme Reis-de-Oliveira; Karina Bispo Dos Santos; Daniel A Toledo-Teixeira; Pierina Lorencini Parise; Matheus Cavalheiro Martini; Rafael Elias Marques; Helison R Carmo; Alexandre Borin; Laís Durço Coimbra; Vinícius O Boldrini; Natalia S Brunetti; Andre S Vieira; Eli Mansour; Raisa G Ulaf; Ana F Bernardes; Thyago A Nunes; Luciana C Ribeiro; Andre C Palma; Marcus V Agrela; Maria Luiza Moretti; Andrei C Sposito; Fabrício Bíscaro Pereira; Licio Augusto Velloso; Marco Aurélio Ramirez Vinolo; André Damasio; José Luiz Proença-Módena; Robson Francisco Carvalho; Marcelo A Mori; Daniel Martins-de-Souza; Helder I Nakaya; Alessandro S Farias; Pedro M Moraes-Vieira
Journal:  Cell Metab       Date:  2020-07-17       Impact factor: 27.287

2.  Impact of SARS-CoV-2 infection on the recovery of peripheral blood mononuclear cells by density gradient.

Authors:  Maria D I Manunta; Giuseppe Lamorte; Francesca Ferrari; Elena Trombetta; Mario Tirone; Cristiana Bianco; Alessandra Cattaneo; Luigi Santoro; Guido Baselli; Manuela Brasca; Mahnoosh Ostadreza; Elisa Erba; Andrea Gori; Alessandra Bandera; Laura Porretti; Luca V C Valenti; Daniele Prati
Journal:  Sci Rep       Date:  2021-03-01       Impact factor: 4.379

3.  Altered bioenergetics and mitochondrial dysfunction of monocytes in patients with COVID-19 pneumonia.

Authors:  Lara Gibellini; Sara De Biasi; Annamaria Paolini; Rebecca Borella; Federica Boraldi; Marco Mattioli; Domenico Lo Tartaro; Lucia Fidanza; Alfredo Caro-Maldonado; Marianna Meschiari; Vittorio Iadisernia; Erica Bacca; Giovanni Riva; Luca Cicchetti; Daniela Quaglino; Giovanni Guaraldi; Stefano Busani; Massimo Girardis; Cristina Mussini; Andrea Cossarizza
Journal:  EMBO Mol Med       Date:  2020-11-05       Impact factor: 12.137

4.  Impaired Cytotoxic Response in PBMCs From Patients With COVID-19 Admitted to the ICU: Biomarkers to Predict Disease Severity.

Authors:  Lorena Vigón; Daniel Fuertes; Javier García-Pérez; Montserrat Torres; Sara Rodríguez-Mora; Elena Mateos; Magdalena Corona; Adolfo J Saez-Marín; Rosa Malo; Cristina Navarro; María Aranzazu Murciano-Antón; Miguel Cervero; José Alcamí; Valentín García-Gutiérrez; Vicente Planelles; María Rosa López-Huertas; Mayte Coiras
Journal:  Front Immunol       Date:  2021-05-26       Impact factor: 7.561

5.  SARS-CoV-2 infection paralyzes cytotoxic and metabolic functions of the immune cells.

Authors:  Yogesh Singh; Christoph Trautwein; Rolf Fendel; Naomi Krickeberg; Georgy Berezhnoy; Rosi Bissinger; Stephan Ossowski; Madhuri S Salker; Nicolas Casadei; Olaf Riess
Journal:  Heliyon       Date:  2021-05-28

6.  Deficiency of antioxidants and increased oxidative stress in COVID-19 patients: A cross-sectional comparative study in Jigawa, Northwestern Nigeria.

Authors:  Yahaya Muhammad; Yamuna Aminu Kani; Sani Iliya; Jafaru Bunza Muhammad; Abubakar Binji; Abdurrahman El-Fulaty Ahmad; Muhd Bashir Kabir; Kabir Umar Bindawa; Armaya'u Ahmed
Journal:  SAGE Open Med       Date:  2021-02-01

7.  Metabolic programs define dysfunctional immune responses in severe COVID-19 patients.

Authors:  Elizabeth A Thompson; Katherine Cascino; Alvaro A Ordonez; Weiqiang Zhou; Ajay Vaghasia; Anne Hamacher-Brady; Nathan R Brady; Im-Hong Sun; Rulin Wang; Avi Z Rosenberg; Michael Delannoy; Richard Rothman; Katherine Fenstermacher; Lauren Sauer; Kathyrn Shaw-Saliba; Evan M Bloch; Andrew D Redd; Aaron A R Tobian; Maureen Horton; Kellie Smith; Andrew Pekosz; Franco R D'Alessio; Srinivasan Yegnasubramanian; Hongkai Ji; Andrea L Cox; Jonathan D Powell
Journal:  Cell Rep       Date:  2021-02-26       Impact factor: 9.423

Review 8.  Mitochondrial Modulations, Autophagy Pathways Shifts in Viral Infections: Consequences of COVID-19.

Authors:  Shailendra Pratap Singh; Salomon Amar; Pinky Gehlot; Sanjib K Patra; Navjot Kanwar; Abhinav Kanwal
Journal:  Int J Mol Sci       Date:  2021-07-30       Impact factor: 5.923
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