| Literature DB >> 35838970 |
Maemu P Gededzha1,2, Sarika Jugwanth3,4, Nakampe Mampeule3,4, Nontobeko Zwane3,4, Anura David5, Lesley Scott4,5, Wendy Stevens4,5, Elizabeth S Mayne3,4,6.
Abstract
Severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) has been identified as the causative agent of COVID-19. Accurate detection of SARS-CoV-2 infection is not only important for management of infected individuals but also to break the chain of transmission. Although the polymerase chain reaction (PCR) is the gold standard for diagnosis of acute SARS-CoV-2 infection, there are a number of limitations of these assays, which include the inability to detect past infection and decline in sensitivity 14 days post-symptom onset. There are several serology tests developed for the detection of SARS-CoV-2 antibodies including high-throughput serology platforms and lateral flow immunoassays. These tests should be evaluated for their performance to meet local regulations acceptance criteria. To optimize the diagnostic algorithm for SARS-CoV-2, this protocol describes the evaluation of serological antibody testing using various automated serology platforms and lateral flow immunoassays. This protocol was evaluated in both serum and plasma samples. The sample preparation, procedure, and data analysis are described. The protocol can be adapted for any serological testing.Entities:
Keywords: Automated serology Platform; Lateral flow immunoassay; Precision; SARS-CoV-2 serology test
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Year: 2022 PMID： 35838970 DOI： 10.1007/978-1-0716-2395-4_23
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745