Xueqiang Feng1, Fuwang Yao2, Yuqin Lang3, Zhibin Cao4. 1. Department of Vascular Intervention, Affiliated Hospital of Weifang Medical University Weifang, Shandong Province, China. 2. Department of Anesthesiology, Affiliated Hospital of Weifang Medical University Weifang, Shandong Province, China. 3. Department of Endoscopic Outpatient Operating Room, Affiliated Hospital of Weifang Medical University Weifang, Shandong Province, China. 4. Department of Endocrinology, Affiliated Hospital of Weifang Medical University Weifang, Shandong Province, China.
Abstract
OBJECTIVE: Atherosclerosis (AS) is a chronic inflammatory disease with high mortality. miRNAs perform a vital role in its development. This study aimed to discuss the effect of miR-145 in AS occurrence and development. METHODS: The effects of miR-145 mimics and inhibitors on IL-6, IL-1β and TNF-α expressions were assessed by qRT-PCR and ELISA. CCK-8 was applied to examine the influence of miR-145 on macrophage proliferation. The influence of miR-145 on the QKI transcriptional activity was analyzed using luciferase reporter gene assy. RESULTS: Overexpression of miR-145 could enhance the expression of IL-6, IL-1β, and TNF-α. Down-regulation of miR-145 could inhibit the proliferation of macrophages and the expression level of inflammatory cytokines. The effect of miR-145 inhibitor on the expression of inflammatory factors was partially reversed by interfering with the transcription of QKI with siRNA. CONCLUSION: miR-145 regulates the inflammatory response induced by macrophage activation through targeting QKI. It provides a means for AS targeted therapy. AJTR
OBJECTIVE: Atherosclerosis (AS) is a chronic inflammatory disease with high mortality. miRNAs perform a vital role in its development. This study aimed to discuss the effect of miR-145 in AS occurrence and development. METHODS: The effects of miR-145 mimics and inhibitors on IL-6, IL-1β and TNF-α expressions were assessed by qRT-PCR and ELISA. CCK-8 was applied to examine the influence of miR-145 on macrophage proliferation. The influence of miR-145 on the QKI transcriptional activity was analyzed using luciferase reporter gene assy. RESULTS: Overexpression of miR-145 could enhance the expression of IL-6, IL-1β, and TNF-α. Down-regulation of miR-145 could inhibit the proliferation of macrophages and the expression level of inflammatory cytokines. The effect of miR-145 inhibitor on the expression of inflammatory factors was partially reversed by interfering with the transcription of QKI with siRNA. CONCLUSION: miR-145 regulates the inflammatory response induced by macrophage activation through targeting QKI. It provides a means for AS targeted therapy. AJTR
Authors: Wenwen Wang; Dongsheng Zhai; Yongquan Bai; Ke Xue; Lele Deng; Lirong Ma; Tianshu Du; Zicheng Ye; Di Qu; An Xiang; Guo Chen; Yi Zhao; Li Wang; Zifan Lu Journal: Cell Death Discov Date: 2021-03-23
Authors: Ruben G de Bruin; Lily Shiue; Jurriën Prins; Hetty C de Boer; Anjana Singh; W Samuel Fagg; Janine M van Gils; Jacques M G J Duijs; Sol Katzman; Adriaan O Kraaijeveld; Stefan Böhringer; Wai Y Leung; Szymon M Kielbasa; John P Donahue; Patrick H J van der Zande; Rick Sijbom; Carla M A van Alem; Ilze Bot; Cees van Kooten; J Wouter Jukema; Hilde Van Esch; Ton J Rabelink; Hilal Kazan; Erik A L Biessen; Manuel Ares; Anton Jan van Zonneveld; Eric P van der Veer Journal: Nat Commun Date: 2016-03-31 Impact factor: 14.919