Ling Fang1, Xianyi Lu2, Chengjun Cui1, Qifeng Shi3, Haojue Wang2. 1. Department of Dermatology, Xishan People's Hospital of Wuxi City, Wuxi Branch of Zhongda Hospital Southeast University Wuxi 214105, Jiangsu, China. 2. Department of Obstetrics and Gynecology, Xishan People's Hospital of Wuxi City, Wuxi Branch of Zhongda Hospital Southeast University Wuxi 214105, Jiangsu, China. 3. Department of Pathology, Xishan People's Hospital of Wuxi City, Wuxi Branch of Zhongda Hospital Southeast University Wuxi 214105, Jiangsu, China.
Abstract
OBJECTIVE: Trichomoniasis is a common sexually-transmitted disease that is associated with increased perinatal morbidity and human immunodeficiency virus (HIV) transmission. This study aimed to develop a Metronidazole-loaded nanoparticulate thermoreversible gel for gynecological infection of Trichomonas vaginalis (T. vaginalis). METHODS: The optimized nanoparticulate formulation was used in thermoreversible gel and characterized for physico-chemical properties, antiparasitic activity, and in vivo efficacy in the BALB/c mouse model. RESULT: A nearly threefold rise in antiparasitic activity of the optimized formulation was observed as compared to that of regular gel. Formulation F5 successfully cured the trichomoniasis within 3 days, while regular gel and pure Metronidazole (MTDZ) failed to cure this infection (P<0.05). CONCLUSION: The present investigation confirms the ability of thermoreversible gel containing nanoparticulate metronidazole againstthe infection by T. vaginalis. The developed gel could be an alternative to the existing drug delivery system for the treatment of trichomoniasis. AJTR
OBJECTIVE: Trichomoniasis is a common sexually-transmitted disease that is associated with increased perinatal morbidity and human immunodeficiency virus (HIV) transmission. This study aimed to develop a Metronidazole-loaded nanoparticulate thermoreversible gel for gynecological infection of Trichomonas vaginalis (T. vaginalis). METHODS: The optimized nanoparticulate formulation was used in thermoreversible gel and characterized for physico-chemical properties, antiparasitic activity, and in vivo efficacy in the BALB/c mouse model. RESULT: A nearly threefold rise in antiparasitic activity of the optimized formulation was observed as compared to that of regular gel. Formulation F5 successfully cured the trichomoniasis within 3 days, while regular gel and pure Metronidazole (MTDZ) failed to cure this infection (P<0.05). CONCLUSION: The present investigation confirms the ability of thermoreversible gel containing nanoparticulate metronidazole againstthe infection by T. vaginalis. The developed gel could be an alternative to the existing drug delivery system for the treatment of trichomoniasis. AJTR