| Literature DB >> 35836071 |
Mitsuru Hattori1, Yukino Itoh2, Takeharu Nagai3,4.
Abstract
Bioluminescent indicators facilitate determination of bioactive molecules in blood samples with high sensitivity. Using a bright luciferase, its bioluminescence (BL) can be easily detected by conventional light sensing devices. In this chapter, we describe a protocol to measure bioactive molecules in blood by taking the BL images with a smartphone camera. We exemplify the measurement of unconjugated bilirubin (UCBR) concentration in the blood of mice using a ratiometric bioluminescent UCBR indicator, BABI (bilirubin assessment with a bioluminescent indicator), and a smartphone camera. We show the UCBR concentration is easily determined through measuring the variance in the BL color with a smartphone camera. This method provides a practical method to lead to future point-of-care diagnosis with quick and simple procedures.Entities:
Keywords: Bioluminescence; Camera; Diagnosis; Indicator; Smartphone; Unconjugated bilirubin (UCBR)
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Year: 2022 PMID： 35836071 DOI： 10.1007/978-1-0716-2473-9_16
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745