Analysis of the subcellular location of lncRNA SLC16A1-AS1 and its interaction with premature miR-5088-5p in oral squamous cell carcinoma.

SLC16A1-AS1 is a newly identified lncRNA with different roles in different cancers. MiR-5088-5p is an oncogenic miRNA in breast cancer. However, their participation in oral squamous cell carcinoma (OSCC) is unknown. We predicted the interaction between SLC16A1-AS1 and miR-5088-5p, and this study was carried out to explore the crosstalk between them in OSCC. A total of 56 OSCC patients donated OSCC and paired non-tumor tissues, which were used to detect the differential expression of SLC16A1-AS1 and miR-5088-5p (mature and premature). Analysis of the subcellular location of SLC16A1-AS1 in OSCC cells and its direct interaction with premature miR-5088-5p was performed with cellular fractionation assay and RNA pull-down assay, respectively. The involvement of SLC16A1-AS1 in miR-5088-5p maturation was studied with overexpression assay. BrdU assay was performed to detect cell proliferation after transfection. OSCC tissue samples exhibited decreased expression levels of SLC16A1-AS1 and premature miR-5088-5p, but increased the expression levels of mature miR-5088-5p. SLC16A1-AS1 was detected in both nucleus and cytoplasm samples of OSCC cells and its direct interaction with premature miR-5088-5p was confirmed. Overexpression of SLC16A1-AS1 in OSCC cells resulted in inhibited maturation of miR-5088-5p. SLC16A1-AS1 suppressed the enhancing effects of miR-5088-5p on cell proliferation. SLC16A1-AS1 was downregulated in OSCC and it may inhibit cell proliferation by suppressing maturation of miR-5088-5p.