Vinícius Taioqui Pelá1, Leonardo Brito2, Even Akemi Taira2, Flávio Henrique-Silva1, Joana Claudio Pieretti3, Amedea Barozzi Seabra3, Cristiane de Almeida Baldini Cardoso4, Eduardo Pereira de Souza1, Sonia Groisman5, Marcela Charantola Rodrigues6, Adrian Lussi7, Thiago Saads Carvalho8, Marília Afonso Rabelo Buzalaf9. 1. Department of Genetics and Evolution, São Carlos Federal University, São Carlos, São Paulo, Brazil. 2. Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil. 3. Center for Natural and Human Sciences, Federal University of ABC, Santo André, São Paulo, Brazil. 4. Postgraduate Department, Cruzeiro do Sul University, São Paulo, São Paulo, Brazil. 5. Federal University of Rio de Janeiro, Rio de Janeiro, Brazil. 6. Municipal University of São Caetano Do Sul (USCS), São Caetano do Sul, São Paulo, Brazil. 7. Department of Operative Dentistry and Periodontology, University Medical Centre, Freiburg, Germany. 8. Department of Restorative, Preventive and Pediatric Dentistry, School of Dental Medicine, University of Bern, Bern, Switzerland. 9. Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil. mbuzalaf@fob.usp.br.
Abstract
OBJECTIVE: This study evaluated the preventive effect of a chitosan gel containing CaneCPI-5 against enamel erosion and erosion + abrasion in situ. METHODS: Sixteen volunteers participated in a crossover, double-blind protocol, comprising 4 phases: (1) no treatment (Nt); (2) chitosan gel (Cg); (3) chitosan gel + 12,300 ppm NaF (Cg + NaF); and (4) chitosan gel + 0.1 mg/mL CaneCPI-5 (Cg + Cane). Volunteers wore an appliance containing 4 specimens. Once/day, they applied the gel (except for Nt) (4 min/specimen). Erosive challenges were performed extra-orally (0.1% citric acid, 90 s, 4 × /day; ERO). Specimens were also abraded (toothbrush, 15 s/specimen, 2 × /day; ERO + ABR). Enamel wear was assessed by profilometry and relative surface reflection intensity (%SRI). Two-way RM-ANOVA/Sidak's tests and Spearman's correlation were used (p < 0.05). RESULTS: For profilometry, ERO + ABR promoted significantly greater wear when compared with ERO. There was a significant difference among all treatments. The lowest enamel loss occurred for Cg + Cane, followed by Cg + NaF, Cg, and Nt (p < 0.05). The %SRI was significantly lower for ERO + ABR when compared to ERO, only for the Nt group. The greatest %SRI was found for the Cg + NaF and Cg + Cane groups, which did not differ significantly, regardless of the conditions. The lowest %SRI was found for the Nt and Cg groups, which did not differ from each other, regardless of the conditions. The Nt group did not differ significantly from the Cg + NaF (ERO). There was a significant correlation between both analyses. CONCLUSION: The incorporation of CaneCPI-5 in the chitosan gel prevented erosive wear in situ. CLINICAL RELEVANCE: These results open a new perspective for the use of CaneCPI-5 in other application vehicles, such as chitosan gel.
OBJECTIVE: This study evaluated the preventive effect of a chitosan gel containing CaneCPI-5 against enamel erosion and erosion + abrasion in situ. METHODS: Sixteen volunteers participated in a crossover, double-blind protocol, comprising 4 phases: (1) no treatment (Nt); (2) chitosan gel (Cg); (3) chitosan gel + 12,300 ppm NaF (Cg + NaF); and (4) chitosan gel + 0.1 mg/mL CaneCPI-5 (Cg + Cane). Volunteers wore an appliance containing 4 specimens. Once/day, they applied the gel (except for Nt) (4 min/specimen). Erosive challenges were performed extra-orally (0.1% citric acid, 90 s, 4 × /day; ERO). Specimens were also abraded (toothbrush, 15 s/specimen, 2 × /day; ERO + ABR). Enamel wear was assessed by profilometry and relative surface reflection intensity (%SRI). Two-way RM-ANOVA/Sidak's tests and Spearman's correlation were used (p < 0.05). RESULTS: For profilometry, ERO + ABR promoted significantly greater wear when compared with ERO. There was a significant difference among all treatments. The lowest enamel loss occurred for Cg + Cane, followed by Cg + NaF, Cg, and Nt (p < 0.05). The %SRI was significantly lower for ERO + ABR when compared to ERO, only for the Nt group. The greatest %SRI was found for the Cg + NaF and Cg + Cane groups, which did not differ significantly, regardless of the conditions. The lowest %SRI was found for the Nt and Cg groups, which did not differ from each other, regardless of the conditions. The Nt group did not differ significantly from the Cg + NaF (ERO). There was a significant correlation between both analyses. CONCLUSION: The incorporation of CaneCPI-5 in the chitosan gel prevented erosive wear in situ. CLINICAL RELEVANCE: These results open a new perspective for the use of CaneCPI-5 in other application vehicles, such as chitosan gel.
Authors: Nadine Schlueter; Bennett T Amaechi; David Bartlett; Marília Afonso Rabelo Buzalaf; Thiago Saads Carvalho; Carolina Ganss; Anderson T Hara; Marie-Charlotte D N J M Huysmans; Adrian Lussi; Rebecca Moazzez; Alexandre Rezende Vieira; Nicola X West; Annette Wiegand; Alix Young; Frank Lippert Journal: Caries Res Date: 2019-10-14 Impact factor: 4.056
Authors: A C Santiago; Z N Khan; M C Miguel; C C Gironda; A Soares-Costa; V T Pelá; A L Leite; J M Edwardson; M A R Buzalaf; F Henrique-Silva Journal: J Dent Res Date: 2017-06-12 Impact factor: 6.116
Authors: Sávio José Cardoso Bezerra; Ítallo Emídio Lira Viana; Idalina Vieira Aoki; Maria Angela Pita Sobral; Alessandra Buhler Borges; Anderson T Hara; Taís Scaramucci Journal: J Mater Sci Mater Med Date: 2021-07-01 Impact factor: 3.896