Literature DB >> 3582751

Bubble free cell culture aeration with porous moving membranes.

J Lehmann, G W Piehl, R Schulz.   

Abstract

For the cultivation of mammalian cells a new aeration system was developed. Its construction with hydrophobic porous Accurel hollow fiber membranes and the use in reactors with 1 and 20 l reaction volume will be presented and introduced by means of the cultivation of a genetically manipulated mouse cell line. The mass transfer capacity of this system is demonstrated as a function of gas flow rates through the membrane and of the membrane movement.

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Year:  1987        PMID： 3582751

Source DB:  PubMed          Journal:  Dev Biol Stand        ISSN： 0301-5149


  12 in total

Review 1.  Serum-free medium for fermentor cultures of hybridomas.

Authors:  O W Merten; J Litwin
Journal:  Cytotechnology       Date:  1991-01       Impact factor: 2.058

2.  High density microcarrier culture with a new device which allows oxygenation and perfusion of microcarrier cultures.

Authors:  M Reiter; F Weigang; W Ernst; H W Katinger
Journal:  Cytotechnology       Date:  1990-01       Impact factor: 2.058

3.  Scale up cultivation of primary human umbilical vein endothelial cells on microcarriers from spinner vessels to bioreactor fermentation.

Authors:  S Duvar; J Müthing; H Mohr; J Lehmann
Journal:  Cytotechnology       Date:  1996-01       Impact factor: 2.058

4.  Lysis-free separation of hybridoma cells by continuous disc stack centrifugation.

Authors:  H Tebbe; D Lütkemeyer; F Gudermann; R Heidemann; J Lehmann
Journal:  Cytotechnology       Date:  1996-01       Impact factor: 2.058

5.  Improving bioreactor cultivation conditions for sensitive cell lines by dynamic membrane aeration.

Authors:  Björn Frahm; Helmut Brod; Uwe Langer
Journal:  Cytotechnology       Date:  2009-03-25       Impact factor: 2.058

6.  Serum-free production of a chimeric E-selectin-IgG protein from 1 to 100 l scale: Repeated batch cultivation versus continuous spin filter perfusion.

Authors:  Andreas Werner; Dirk Lütkemeyer; Iris Poggendorf; Jürgen Lehmann; Johannes Müthing
Journal:  Cytotechnology       Date:  2002-01       Impact factor: 2.058

7.  The Super-Spinner: a low cost animal cell culture bioreactor for the CO2 incubator.

Authors:  R Heidemann; U Riese; D Lütkemeyer; H Büntemeyer; J Lehmann
Journal:  Cytotechnology       Date:  1994       Impact factor: 2.058

8.  Glycoprotein production for structure analysis with stable, glycosylation mutant CHO cell lines established by fluorescence-activated cell sorting.

Authors:  Sonja Wilke; Joern Krausze; Manfred Gossen; Lothar Groebe; Volker Jäger; Ermanno Gherardi; Joop van den Heuvel; Konrad Büssow
Journal:  Protein Sci       Date:  2010-06       Impact factor: 6.725

9.  Agitation, aeration and perfusion modules for cell culture bioreactors.

Authors:  C Fenge; C Klein; C Heuer; U Siegel; E Fraune
Journal:  Cytotechnology       Date:  1993       Impact factor: 2.058

10.  Oxygenation of intensive cell-culture system.

Authors:  A N Emery; D C Jan; M al-Rubeai
Journal:  Appl Microbiol Biotechnol       Date:  1995-11       Impact factor: 4.813
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