Literature DB >> 3582673

The efficiency of dNTP complex formation with human placenta DNA polymerase alpha as demonstrated by affinity modification.

S V Doronin, O I Lavrik, G A Nevinsky, V N Podust.   

Abstract

The interaction of deoxyribonucleoside 5'-mono-, di- and triphosphates with human placenta DNA polymerase alpha was examined. Dissociation constants of enzyme complex formation with dNMP, dNDP and dNTP were determined from the data on enzyme affinity modification by imidazolide of dTMP. The basic role of the primary template-primer interaction with the enzyme in dNTP complex formation is shown. The template-dependent nucleotide interaction does not occur in the case of dNMP and dNDP in comparison with dNTP. The significant contribution of the gamma-phosphate of dNTP in this process is demonstrated.

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Year:  1987        PMID： 3582673     DOI： 10.1016/0014-5793(87)80693-2

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  4 in total

1.  Site-directed mutagenesis in the fingers subdomain of HIV-1 reverse transcriptase reveals a specific role for the beta3-beta4 hairpin loop in dNTP selection.

Authors:  Scott J Garforth; Tae Woo Kim; Michael A Parniak; Eric T Kool; Vinayaka R Prasad
Journal:  J Mol Biol       Date:  2006-09-27       Impact factor: 5.469

Review 2.  Uracil-DNA glycosylase: Structural, thermodynamic and kinetic aspects of lesion search and recognition.

Authors:  Dmitry O Zharkov; Grigory V Mechetin; Georgy A Nevinsky
Journal:  Mutat Res       Date:  2009-11-10       Impact factor: 2.433

3.  Thermodynamic, kinetic and structural basis for recognition and repair of abasic sites in DNA by apurinic/apyrimidinic endonuclease from human placenta.

Authors:  Natalia G Beloglazova; Oleg O Kirpota; Konstantin V Starostin; Alexander A Ishchenko; Vitaly I Yamkovoy; Dmitry O Zharkov; Kenneth T Douglas; Georgy A Nevinsky
Journal:  Nucleic Acids Res       Date:  2004-09-30       Impact factor: 16.971

4.  Utilization of a deoxynucleoside diphosphate substrate by HIV reverse transcriptase.

Authors:  Scott J Garforth; Michael A Parniak; Vinayaka R Prasad
Journal:  PLoS One       Date:  2008-04-30       Impact factor: 3.240
  4 in total

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