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Literature DB >> 3582489

Monoclonal anti-allotype antibody towards BALB/c IgM. Analysis of specificity and site of a V-C crossover in recombinant strain BALB-Igh-Va/Igh-Cb.

Abstract

Polyclonal IgM obtained by fractionation of a BALB/c serum pool was used as the immunogen for C57BL/6 mice. Draining lymph nodes from selected animals donated cells for fusion with myeloma Sp-2/0. Fifteen hybridomas were productive, and one (RS-3.1) was cloned and the affinity-purified product analyzed for its reactivity pattern by MOPC 104E-enzyme-linked immunosorbent assay inhibition. Among five BALB/c myelomas only TEPC 183 (IgM) was active, not those belonging to other Ig classes. Among normal sera from 8 mouse strains only those of BALB/c, DBA/2J and CBA/J showed inhibition. The recombinant strain BALB-Igh-Va/Igh-Cb did not react, which shows that its C mu stems from parental strain C57BL/6, and that therefore the recombination event had occurred 5' of this gene.

Entities: Disease Gene Species

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Year: 1987 PMID： 3582489 DOI： 10.1002/eji.1830170527

Source DB: PubMed Journal: Eur J Immunol ISSN： 0014-2980 Impact factor: 5.532

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Cited

25 in total

1. Mice reconstituted with DNA polymerase beta-deficient fetal liver cells are able to mount a T cell-dependent immune response and mutate their Ig genes normally.

Authors: G Esposito; G Texido; U A Betz; H Gu; W Müller; U Klein; K Rajewsky
Journal: Proc Natl Acad Sci U S A Date: 2000-02-01 Impact factor: 11.205

2. Tumorigenesis mediated by an antigen receptor.

Authors: H M Jäck; G Beck-Engeser; G Lee; D Wofsy; M Wabl
Journal: Proc Natl Acad Sci U S A Date: 1992-09-15 Impact factor: 11.205

3. Peritoneal B cells regulate the numbers of allotype-matched pre-B and B cells in bone marrow.

Authors: M A Marcos; A Sundblad; E Malenchère; A Coutinho
Journal: Proc Natl Acad Sci U S A Date: 1991-11-15 Impact factor: 11.205

4. Immunoglobulin D-deficient mice can mount normal immune responses to thymus-independent and -dependent antigens.

Authors: L Nitschke; M H Kosco; G Köhler; M C Lamers
Journal: Proc Natl Acad Sci U S A Date: 1993-03-01 Impact factor: 11.205

5. Development of B-lineage cells in the bone marrow of scid/scid mice following the introduction of functionally rearranged immunoglobulin transgenes.

Authors: M Reichman-Fried; R R Hardy; M J Bosma
Journal: Proc Natl Acad Sci U S A Date: 1990-04 Impact factor: 11.205

Monoclonal anti-allotype antibody towards BALB/c IgM. Analysis of specificity and site of a V-C crossover in recombinant strain BALB-Igh-Va/Igh-Cb.

1. Mice reconstituted with DNA polymerase beta-deficient fetal liver cells are able to mount a T cell-dependent immune response and mutate their Ig genes normally.

2. Tumorigenesis mediated by an antigen receptor.

3. Peritoneal B cells regulate the numbers of allotype-matched pre-B and B cells in bone marrow.

4. Immunoglobulin D-deficient mice can mount normal immune responses to thymus-independent and -dependent antigens.

5. Development of B-lineage cells in the bone marrow of scid/scid mice following the introduction of functionally rearranged immunoglobulin transgenes.

6. B-cell maturation in chimaeric mice deficient for the heat stable antigen (HSA/mouse CD24).

7. Modulation of B-cell abnormalities in lupus-prone (NZB x NZW)F1 mice by normal bone marrow-derived B-lineage cells.

8. B cell tolerance in mice transgenic for anti-CD8 immunoglobulin mu chain.

9. Enrichment and characterization of uncommitted B-cell precursors from fetal liver at day 12 of gestation.

10. Elimination of CD8+ thymocytes in transgenic mice expressing an anti-Lyt2.2 immunoglobulin heavy chain gene.