Literature DB >> 35821489

Dual-Luciferase-Based Fast and Sensitive Detection of Malaria Hypnozoites for the Discovery of Anti-relapse Compounds.

Annemarie M Voorberg-van der Wel1, Anne-Marie Zeeman1, Ivonne G Nieuwenhuis1, Nicole M van der Werff1, Clemens H M Kocken2.   

Abstract

Malaria hypnozoites are dormant parasite stages that reside inside hepatocytes. Upon activation, these stages can resume growth, causing new episodes of blood stage malaria infection. This chapter describes a fast and sensitive protocol for the detection of bioluminescent (BL) hypnozoites in vitro. Using transgenic Plasmodium cynomolgi parasites that differentially express the BL reporter proteins firefly luciferase and the ultrabright NanoLuc, hypnozoites can be distinguished from liver stage schizonts. This robust method sets the stage for implementation in large-scale drug screening platforms with the aim to find new compounds that eliminate hypnozoites.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Bioluminescence (BL); Drug screening; Dual; Firefly luciferase; Hepatocytes; Hypnozoites; Liver; Liver stages; Malaria; Monkeys; NanoLuc; Plasmodium cynomolgi; Sporozoites

Mesh：

Substances：

Year:  2022        PMID： 35821489     DOI： 10.1007/978-1-0716-2453-1_31

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  4 in total

1.  High yield preparation of isolated human adult hepatocytes by enzymatic perfusion of the liver.

Authors:  C Guguen-Guillouzo; J P Campion; P Brissot; D Glaise; B Launois; M Bourel; A Guillouzo
Journal:  Cell Biol Int Rep       Date:  1982-06

2.  Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate.

Authors:  Mary P Hall; James Unch; Brock F Binkowski; Michael P Valley; Braeden L Butler; Monika G Wood; Paul Otto; Kristopher Zimmerman; Gediminas Vidugiris; Thomas Machleidt; Matthew B Robers; Hélène A Benink; Christopher T Eggers; Michael R Slater; Poncho L Meisenheimer; Dieter H Klaubert; Frank Fan; Lance P Encell; Keith V Wood
Journal:  ACS Chem Biol       Date:  2012-08-30       Impact factor: 5.100

3.  Towards an in vitro model of Plasmodium hypnozoites suitable for drug discovery.

Authors:  Laurent Dembele; Audrey Gego; Anne-Marie Zeeman; Jean-François Franetich; Olivier Silvie; Armelle Rametti; Roger Le Grand; Nathalie Dereuddre-Bosquet; Robert Sauerwein; Geert-Jan van Gemert; Jean-Christophe Vaillant; Alan W Thomas; Georges Snounou; Clemens H M Kocken; Dominique Mazier
Journal:  PLoS One       Date:  2011-03-31       Impact factor: 3.240

4.  A dual fluorescent Plasmodium cynomolgi reporter line reveals in vitro malaria hypnozoite reactivation.

Authors:  Annemarie M Voorberg-van der Wel; Anne-Marie Zeeman; Ivonne G Nieuwenhuis; Nicole M van der Werff; Els J Klooster; Onny Klop; Lars C Vermaat; Devendra Kumar Gupta; Laurent Dembele; Thierry T Diagana; Clemens H M Kocken
Journal:  Commun Biol       Date:  2020-01-03
  4 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.