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Literature DB >> 35821481

Non-invasive In Vivo Tracking of Mammalian Cells Stably Expressing Firefly Luciferase.

Abstract

Firefly luciferase (FLuc)-based in vivo optical imaging technology exerts the non-invasive monitoring of transplanted cells in experimental animal models. This chapter introduces an established cell line that stably expresses a retrovirus-delivered FLuc protein gene. The stable expression does not affect the cell morphology, proliferation, migration, and invasion abilities of the parental cells. After implantation, the bioluminescence signal of FLuc cells truly reflects cell proliferation and survival in vivo, which can provide a reliable method for dynamic detection of in vivo cell transplantation.

Entities: Chemical

Keywords: Bioluminescence imaging (BLI); Firefly luciferase (FLuc); In vivo cell tracking Retrovirus; Stable cell line

Mesh：

Substances：
Luciferases, Firefly

Year: 2022 PMID： 35821481 DOI： 10.1007/978-1-0716-2453-1_23

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

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References

11 in total

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Non-invasive In Vivo Tracking of Mammalian Cells Stably Expressing Firefly Luciferase.

Review 1. In vivo cell tracking with bioluminescence imaging.

Review 2. Noninvasive cell-tracking methods.

3. Using Bioengineered Bioluminescence to Track Stem Cell Transplantation In Vivo.

4. Tracing and Characterizing the Development of Transplanted Female Germline Stem Cells In Vivo.

5. Comparison of cell-labeling methods with ¹²⁴I-FIAU and ⁶⁴Cu-PTSM for cell tracking using chronic myelogenous leukemia cells expressing HSV1-tk and firefly luciferase.

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