| Literature DB >> 35821476 |
Desarey Morales Vasquez1, Kevin Chiem1, Chengjin Ye1, Luis Martinez-Sobrido2,3.
Abstract
Reporter-expressing recombinant severe acute respiratory syndrome coronavirus 2 (rSARS-CoV-2) represents an excellent tool to understand the biology of and ease studying viral infections in vitro and in vivo. The broad range of applications of reporter-expressing recombinant viruses is due to the facilitated expression of fluorescence or bioluminescence readouts. In this chapter, we describe a detailed protocol on the generation of rSARS-CoV-2 expressing Venus, mCherry, and NLuc that represents a valid surrogate to track viral infections.Entities:
Keywords: Bioluminescence (BL); Coronavirus; Coronavirus disease 2019 (COVID-19); Fluorescence (FL); NanoLuc (NLuc); Recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2); Reporter genes; Reporter virus; Venus; mCherry
Mesh：
Year: 2022 PMID： 35821476 DOI： 10.1007/978-1-0716-2453-1_18
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745