Kai Pi1,2, Wen Luo1,2, Yin Huang3,4, Renxiang Liu5,6, Zejun Mo1,2,7, Lili Duan1,2,7, Yuzhou Ke1,2, Pingsong Wang1,2,7, Shuaibo Zeng1,2. 1. College of Tobacco, Guizhou University, Huaxi District, Guizhou Province, 550025, Guiyang City, P. R. China. 2. Key Laboratory for Tobacco Quality Research Guizhou Province, Guizhou University, 550025, Guiyang, P. R. China. 3. College of Tobacco, Guizhou University, Huaxi District, Guizhou Province, 550025, Guiyang City, P. R. China. 316452492@qq.com. 4. Key Laboratory for Tobacco Quality Research Guizhou Province, Guizhou University, 550025, Guiyang, P. R. China. 316452492@qq.com. 5. College of Tobacco, Guizhou University, Huaxi District, Guizhou Province, 550025, Guiyang City, P. R. China. rxliu@gzu.edu.cn. 6. Key Laboratory for Tobacco Quality Research Guizhou Province, Guizhou University, 550025, Guiyang, P. R. China. rxliu@gzu.edu.cn. 7. College of Agriculture, Guizhou University, 550025, Guiyang, P. R. China.
Abstract
BACKGROUND: Potassium(K+) plays a vital role in improving the quality of tobacco leaves. However, how to improve the potassium content of tobacco leaves has always been a difficult problem in tobacco planting. K+ content in tobacco hybrid is characterized by heterosis, which can improve the quality of tobacco leaves, but its underlying molecular genetic mechanisms remain unclear. RESULTS: Through a two-year field experiment, G70×GDH11 with strong heterosis and K326×GDH11 with weak heterosis were screened out. Transcriptome analyses revealed that 80.89% and 57.28% of the differentially expressed genes (DEGs) in the strong and weak heterosis combinations exhibited an overdominant expression pattern, respectively. The genes that up-regulated the overdominant expression in the strong heterosis hybrids were significantly enriched in the ion homeostasis. Genes involved in K+ transport (KAT1/2, GORK, AKT2, and KEA3), activity regulation complex (CBL-CIPK5/6), and vacuole (TPKs) genes were overdominant expressed in strong heterosis hybrids, which contributed to K+ homeostasis and heterosis in tobacco leaves. CONCLUSIONS: K+ homeostasis and accumulation in tobacco hybrids were collectively improved. The overdominant expression of K+ transport and homeostasis-related genes conducted a crucial role in the heterosis of K+ content in tobacco leaves.
BACKGROUND: Potassium(K+) plays a vital role in improving the quality of tobacco leaves. However, how to improve the potassium content of tobacco leaves has always been a difficult problem in tobacco planting. K+ content in tobacco hybrid is characterized by heterosis, which can improve the quality of tobacco leaves, but its underlying molecular genetic mechanisms remain unclear. RESULTS: Through a two-year field experiment, G70×GDH11 with strong heterosis and K326×GDH11 with weak heterosis were screened out. Transcriptome analyses revealed that 80.89% and 57.28% of the differentially expressed genes (DEGs) in the strong and weak heterosis combinations exhibited an overdominant expression pattern, respectively. The genes that up-regulated the overdominant expression in the strong heterosis hybrids were significantly enriched in the ion homeostasis. Genes involved in K+ transport (KAT1/2, GORK, AKT2, and KEA3), activity regulation complex (CBL-CIPK5/6), and vacuole (TPKs) genes were overdominant expressed in strong heterosis hybrids, which contributed to K+ homeostasis and heterosis in tobacco leaves. CONCLUSIONS: K+ homeostasis and accumulation in tobacco hybrids were collectively improved. The overdominant expression of K+ transport and homeostasis-related genes conducted a crucial role in the heterosis of K+ content in tobacco leaves.
Authors: Daehwan Kim; Joseph M Paggi; Chanhee Park; Christopher Bennett; Steven L Salzberg Journal: Nat Biotechnol Date: 2019-08-02 Impact factor: 54.908
Authors: Ute Armbruster; L Ruby Carrillo; Kees Venema; Lazar Pavlovic; Elisabeth Schmidtmann; Ari Kornfeld; Peter Jahns; Joseph A Berry; David M Kramer; Martin C Jonikas Journal: Nat Commun Date: 2014-11-13 Impact factor: 14.919
Authors: D V Klopfenstein; Liangsheng Zhang; Brent S Pedersen; Fidel Ramírez; Alex Warwick Vesztrocy; Aurélien Naldi; Christopher J Mungall; Jeffrey M Yunes; Olga Botvinnik; Mark Weigel; Will Dampier; Christophe Dessimoz; Patrick Flick; Haibao Tang Journal: Sci Rep Date: 2018-07-18 Impact factor: 4.379