Literature DB >> 35819763

Electron Tomographic Methods for Studying Organelles of the Murine Chemical Synapse.

Tamara Basta1, Garry P Morgan2, Eileen T O'Toole2, Nalini R Rao3, Jeffrey N Savas4, Michael H B Stowell5,6.   

Abstract

Electron tomography of the chemical synapse provides important architectural information regarding the organization of synaptic organelles including synaptic vesicles, Nissl bodies, and early endosomes. Here, we describe methods for the preparation of select murine brain regions for high-pressure freezing, freeze substitution, and EM tomographic analysis of synaptic structures. The method uses fresh brain slices prepared using a vibratome and biopsy punches to collect specific brain regions of interest suitable for subsequent preservation and EM tomographic imaging.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Brain; Electron microscopy; Electron tomography; Freeze substitution; High-pressure freezing; Murine; Subcellular organelles; Synapse

Mesh：

Year:  2022        PMID： 35819763     DOI： 10.1007/978-1-0716-2209-4_10

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  9 in total

1.  The architecture of active zone material at the frog's neuromuscular junction.

Authors:  M L Harlow; D Ress; A Stoschek; R M Marshall; U J McMahan
Journal:  Nature       Date:  2001-01-25       Impact factor: 49.962

Review 2.  Structure suggests function: the case for synaptic ribbons as exocytotic nanomachines.

Authors:  D Lenzi; H von Gersdorff
Journal:  Bioessays       Date:  2001-09       Impact factor: 4.345

3.  The structural organization of the readily releasable pool of synaptic vesicles.

Authors:  Silvio O Rizzoli; William J Betz
Journal:  Science       Date:  2004-03-26       Impact factor: 47.728

4.  Automated electron microscope tomography using robust prediction of specimen movements.

Authors:  David N Mastronarde
Journal:  J Struct Biol       Date:  2005-10       Impact factor: 2.867

5.  Computer visualization of three-dimensional image data using IMOD.

Authors:  J R Kremer; D N Mastronarde; J R McIntosh
Journal:  J Struct Biol       Date:  1996 Jan-Feb       Impact factor: 2.867

Review 6.  Resolving presynaptic structure by electron tomography.

Authors:  Guy A Perkins; Dakota R Jackson; George A Spirou
Journal:  Synapse       Date:  2015-03-09       Impact factor: 2.562

7.  Pulse-Chase Proteomics of the App Knockin Mouse Models of Alzheimer's Disease Reveals that Synaptic Dysfunction Originates in Presynaptic Terminals.

Authors:  Timothy J Hark; Nalini R Rao; Charlotte Castillon; Tamara Basta; Samuel Smukowski; Huan Bao; Arun Upadhyay; Ewa Bomba-Warczak; Toshihiro Nomura; Eileen T O'Toole; Garry P Morgan; Laith Ali; Takashi Saito; Christelle Guillermier; Takaomi C Saido; Matthew L Steinhauser; Michael H B Stowell; Edwin R Chapman; Anis Contractor; Jeffrey N Savas
Journal:  Cell Syst       Date:  2020-12-15       Impact factor: 10.304

8.  Quantitative analysis of the native presynaptic cytomatrix by cryoelectron tomography.

Authors:  Rubén Fernández-Busnadiego; Benoît Zuber; Ulrike Elisabeth Maurer; Marek Cyrklaff; Wolfgang Baumeister; Vladan Lucic
Journal:  J Cell Biol       Date:  2010-01-11       Impact factor: 10.539

9.  Ultrastructural Imaging of Activity-Dependent Synaptic Membrane-Trafficking Events in Cultured Brain Slices.

Authors:  Cordelia Imig; Francisco José López-Murcia; Lydia Maus; Inés Hojas García-Plaza; Lena Sünke Mortensen; Manuela Schwark; Valentin Schwarze; Julie Angibaud; U Valentin Nägerl; Holger Taschenberger; Nils Brose; Benjamin H Cooper
Journal:  Neuron       Date:  2020-09-28       Impact factor: 17.173
  9 in total

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