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Literature DB >> 35819605

The Use of qPCR to Detect Cryphonectria parasitica in Plants.

Abstract

Cryphonectria parasitica is a fungal pathogen that causes lethal bark necrosis in chestnut. A duplex qPCR allowing detection of the pathogen and its host, Castanea sativa, is described. The method can be used for early detection of the pathogen in chestnut bark tissues with an internal control of false-negative results caused by PCR inhibitors and/or DNA extraction failure. A positive amplification control of qPCR that allows detection of any deviation from a normal qPCR run based on a control chart is also described. As C. parasitica is a regulated pathogen in Europe, the protocol also provides information on the way to collect and handle bark samples to fulfil biosecurity rules.

Entities: Chemical

Keywords: Chestnut blight; Endophyte; Fungal pathogen; Internal amplification control; Positive amplification control; Real-time PCR

Mesh：

Year: 2022 PMID： 35819605 DOI： 10.1007/978-1-0716-2517-0_11

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

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References

6 in total

Review 1. Cryphonectria parasitica, the causal agent of chestnut blight: invasion history, population biology and disease control.

Authors: Daniel Rigling; Simone Prospero
Journal: Mol Plant Pathol Date: 2017-04-24 Impact factor: 5.663

2. A phenol/chloroform-free method to extract nucleic acids from recalcitrant, woody tropical species for gene expression and sequencing.

Authors: François F Barbier; Tinashe G Chabikwa; Muhammad U Ahsan; Stacey E Cook; Rosanna Powell; Milos Tanurdzic; Christine A Beveridge
Journal: Plant Methods Date: 2019-06-05 Impact factor: 4.993

3. Saprophytic Activity and Sporulation of Cryphonectria parasitica on Dead Chestnut Wood in Forests with Naturally Established Hypovirulence.

Authors: S Prospero; M Conedera; U Heiniger; D Rigling
Journal: Phytopathology Date: 2006-12 Impact factor: 4.025

The Use of qPCR to Detect Cryphonectria parasitica in Plants.

Review 1. Cryphonectria parasitica, the causal agent of chestnut blight: invasion history, population biology and disease control.

2. A phenol/chloroform-free method to extract nucleic acids from recalcitrant, woody tropical species for gene expression and sequencing.

3. Saprophytic Activity and Sporulation of Cryphonectria parasitica on Dead Chestnut Wood in Forests with Naturally Established Hypovirulence.

Review 4. A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and Everything.

5. Development of a universal endogenous qPCR control for eukaryotic DNA samples.

6. Using acetone for rapid PCR-amplifiable DNA extraction from recalcitrant woody plant taxa.