| Literature DB >> 35819597 |
Cristina Zamora-Ballesteros1, Reinaldo Pire2, Julio Javier Diez3.
Abstract
Fusarium circinatum is a serious invasive pathogen affecting conifers and causes the disease commonly known as pine pitch canker. Due to the outbreak in European countries, regulations stipulate that Member States must conduct annual official surveys for the fungus on their territory and report the results to the European Commission. Here, we describe the field and laboratory protocols used for the identification and diagnostic of the pathogen.Entities:
Keywords: Agarose gel electrophoresis; Identification; Internal transcribes spacer of the rRNA; PCR amplification; Pine pitch canker; Real-time PCR; Sampling; Sanger sequencing
Mesh:
Year: 2022 PMID: 35819597 DOI: 10.1007/978-1-0716-2517-0_3
Source DB: PubMed Journal: Methods Mol Biol ISSN: 1064-3745