Literature DB >> 3579885

Ultramicro-ELISA for calmodulin and anticalmodulin.

I Wenz, R Reissmann, H Börnig, H Hoppe, G Cumme.   

Abstract

A non-competitive ELISA-technique for the determination of anticalmodulin as well as a sensitive competitive ELISA for calmodulin in 10 microliters samples has been developed. The assay for calmodulin has an inter-assay and intra-assay variability of 18% and 17%, respectively; the limit of detection is 20 pg per assay. The corresponding values for the assay of anticalmodulin are less than 6%, less than 6% and 35 pg, respectively. The methods for the purification of calmodulin and the production of anticalmodulin are also described.

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Year:  1987        PMID： 3579885

Source DB:  PubMed          Journal:  Biomed Biochim Acta        ISSN： 0232-766X


  3 in total

1.  Immunohistochemical localization of calmodulin in normal and psoriatic epidermis.

Authors:  U Wollina; R Klinger; R Wetzker; R Reissmann; B Knopf
Journal:  Arch Dermatol Res       Date:  1989       Impact factor: 3.017

2.  Influence of Ca2+ and Mg2+ on the turnover of the phosphomonoester group of phosphatidylinositol 4-phosphate in human erythrocyte membranes.

Authors:  H Hegewald; E Müller; R Klinger; R Wetzker; H Frunder
Journal:  Biochem J       Date:  1987-05-15       Impact factor: 3.857

3.  Myocardial mRNA content and stability, and enzyme activities of Ca-cycling and aerobic metabolism in canine dilated cardiomyopathies.

Authors:  P J O'Brien; A L Duke; H Shen; R V Shohet
Journal:  Mol Cell Biochem       Date:  1995-01-26       Impact factor: 3.396
  3 in total

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