Literature DB >> 35796956

PIWI-Directed DNA Elimination for Tetrahymena Genetics.

Salman Shehzada1, Kazufumi Mochizuki2.   

Abstract

Piwi-bound small RNAs induce programmed DNA elimination in the ciliated protozoan Tetrahymena. Using the phenomenon called codeletion, this process can be reprogrammed to induce ectopic DNA elimination at basically any given genomic location. Here, we describe the usage of codeletion for genetic studies in Tetrahymena and for investigations of the molecular mechanism of Piwi-directed programmed DNA elimination.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  DNA elimination; Gene knockout; PIWI; Small RNA; Tetrahymena

Mesh：

Substances：

Year:  2022        PMID： 35796956     DOI： 10.1007/978-1-0716-2380-0_3

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  4 in total

1.  Structure of the germline genome of Tetrahymena thermophila and relationship to the massively rearranged somatic genome.

Authors:  Eileen P Hamilton; Aurélie Kapusta; Piroska E Huvos; Shelby L Bidwell; Nikhat Zafar; Haibao Tang; Michalis Hadjithomas; Vivek Krishnakumar; Jonathan H Badger; Elisabet V Caler; Carsten Russ; Qiandong Zeng; Lin Fan; Joshua Z Levin; Terrance Shea; Sarah K Young; Ryan Hegarty; Riza Daza; Sharvari Gujja; Jennifer R Wortman; Bruce W Birren; Chad Nusbaum; Jainy Thomas; Clayton M Carey; Ellen J Pritham; Cédric Feschotte; Tomoko Noto; Kazufumi Mochizuki; Romeo Papazyan; Sean D Taverna; Paul H Dear; Donna M Cassidy-Hanley; Jie Xiong; Wei Miao; Eduardo Orias; Robert S Coyne
Journal:  Elife       Date:  2016-11-28       Impact factor: 8.140

2.  Post-meiotic DNA double-strand breaks occur in Tetrahymena, and require Topoisomerase II and Spo11.

Authors:  Takahiko Akematsu; Yasuhiro Fukuda; Jyoti Garg; Jeffrey S Fillingham; Ronald E Pearlman; Josef Loidl
Journal:  Elife       Date:  2017-06-16       Impact factor: 8.140

3.  Ciliary force-responsive striated fibers promote basal body connections and cortical interactions.

Authors:  Adam W J Soh; Teunis J P van Dam; Alexander J Stemm-Wolf; Andrew T Pham; Garry P Morgan; Eileen T O'Toole; Chad G Pearson
Journal:  J Cell Biol       Date:  2020-01-06       Impact factor: 10.539

4.  Spatial constraints on chromosomes are instrumental to meiotic pairing.

Authors:  Miao Tian; Christiane Agreiter; Josef Loidl
Journal:  J Cell Sci       Date:  2020-11-30       Impact factor: 5.285
  4 in total

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