| Literature DB >> 35793029 |
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Abstract
Entities:
Year: 2022 PMID: 35793029 PMCID: PMC9258693 DOI: 10.1111/jcmm.17446
Source DB: PubMed Journal: J Cell Mol Med ISSN: 1582-1838 Impact factor: 5.295
FIGURE 8Inflammation induced by LPS was mitigated by the inhibition of the JNK pathway in the retina. (A) Representative immunofluorescent images of the blank‐rLV + PBS, blank‐rLV + LPS and shRNA‐SHP‐1‐rLV + LPS groups at 24 h after LPS administration. (B) Representative images of the blank‐rLV + PBS, blank‐rLV + LPS and SHP‐1‐rLV + LPS groups at 24 h after LPS administration. Red: pJNK; grey: GS; blue: DAPI. Scale bar: 50 μm. (C) Representative biomicroscopic images of the blank‐rLV + PBS, blank‐rLV + LPS, blank‐rLV + LPS + SP600125, shRNA‐SHP‐1‐rLV + LPS, shRNA‐SHP‐1‐rLV + LPS + SP600125 groups at 0, 24, 48 and 72 h after LPS treatment. (D) Clinical scores for the five groups described above. One‐way anova followed by Dunnett's test was used. n = 5 per group. *Blank‐rLV + LPS + SP600125 group versus blank‐rLV + LPS group; #shRNA‐SHP‐1‐rLV + LPS + SP600125 group versus shRNA‐SHP‐1‐rLV + LPS group. (E–H) Western blotting analysis of the retinal expression of COX2, IL‐1β and MIP‐1α at 24 h after LPS administration. One‐way anova followed by Dunnett's test was used. n = 3 per group. */ # p < 0.05 and **/# # p < 0.01