Literature DB >> 3578782

Rapid separation of oligomers of polysialic acid by high-performance liquid chromatography.

P C Hallenbeck, F Yu, F A Troy.   

Abstract

A rapid procedure utilizing high-performance liquid chromatography was developed for the separation of homooligomers of sialic acid (N-acetylneuraminic acid). The method utilizes the anion exchanger Mono-Q HR 5/5 and can resolve sialyl oligomers with degrees of polymerization (DP) from 2 to 20 in 25 min. Previous methods required 1 to 9 days. Recoveries are quantitative and the method can be used either analytically to analyze the enzymatic digestion products of polysialic acid or semipreparatively to prepare sialyl oligomers of defined length. The method is potentially useful for analyzing other anionic oligosaccharides.

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Year:  1987        PMID： 3578782     DOI： 10.1016/0003-2697(87)90670-1

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  6 in total

1.  CMP-NeuAc:(NeuAc alpha 2-->8)n (colominic acid) sialyltransferase activity in rat brain and in tumour cells that express polysialic acid on neural cell adhesion molecules.

Authors:  E W Easton; W E Schiphorst; C A Koeleman; R J Michalides; D H Van Den Eijnden
Journal:  Glycoconj J       Date:  1995-12       Impact factor: 2.916

2.  H.p.l.c. of oligo(sialic acids). Application to the determination of the minimal chain length serving as exogenous acceptor in the enzymic synthesis of colominic acid.

Authors:  M A Ferrero; J M Luengo; A Reglero
Journal:  Biochem J       Date:  1991-12-15       Impact factor: 3.857

3.  Production and characterization of a monoclonal antibody (BBH5) directed to ganglioside lactone.

Authors:  B Bouchon; S B Levery; H Clausen; S Hakomori
Journal:  Glycoconj J       Date:  1992-02       Impact factor: 2.916

4.  Polyacrylamide gel electrophoresis of the capsular polysaccharides of Escherichia coli K1 and other bacteria.

Authors:  S Pelkonen; J Häyrinen; J Finne
Journal:  J Bacteriol       Date:  1988-06       Impact factor: 3.490

Review 5.  Current status of meningococcal group B vaccine candidates: capsular or noncapsular?

Authors:  J Diaz Romero; I M Outschoorn
Journal:  Clin Microbiol Rev       Date:  1994-10       Impact factor: 26.132

6.  Differential activities of bacteriophage depolymerase on bacterial polysaccharide: binding is essential but degradation is inhibitory in phage infection of K1-defective Escherichia coli.

Authors:  S Pelkonen; J Aalto; J Finne
Journal:  J Bacteriol       Date:  1992-12       Impact factor: 3.490
  6 in total

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