Literature DB >> 3578763

Gas-phase hydrolysis of proteins and peptides.

N M Meltzer, G I Tous, S Gruber, S Stein.   

Abstract

A simplified gas-phase hydrolysis procedure for proteins and peptides is described. The apparatus consists of a glass vacuum desiccator, a ceramic plate, and a Teflon ring. The method was shown to give reproducible compositions for hydrolysis of human serum albumin and microanalysis of alpha-melanocyte stimulating hormone including the quantitation of as little as one residue of tryptophan. It minimizes sample handling and allows for the simultaneous hydrolysis of a large number of samples.

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Year:  1987        PMID: 3578763     DOI: 10.1016/0003-2697(87)90060-1

Source DB:  PubMed          Journal:  Anal Biochem        ISSN: 0003-2697            Impact factor:   3.365


  12 in total

1.  Functional implications of ribosomal protein L2 in protein biosynthesis as shown by in vivo replacement studies.

Authors:  M Uhlein; W Weglöhner; H Urlaub; B Wittmann-Liebold
Journal:  Biochem J       Date:  1998-04-15       Impact factor: 3.857

2.  Neonatal plasminogen displays altered cell surface binding and activation kinetics. Correlation with increased glycosylation of the protein.

Authors:  J M Edelberg; J J Enghild; S V Pizzo; M Gonzalez-Gronow
Journal:  J Clin Invest       Date:  1990-07       Impact factor: 14.808

3.  Amino acid sequence of the ribosomal protein HS23 from the halophilic Haloarcula marismortui and homology studies to other ribosomal proteins.

Authors:  S Engemann; E Herfurth; U Briesemeister; B Wittmann-Liebold
Journal:  J Protein Chem       Date:  1995-05

4.  Assignment of a single disulphide bridge in human alpha2-antiplasmin: implications for the structural and functional properties.

Authors:  S Christensen; Z Valnickova; I B Thogersen; E H Olsen; J J Enghild
Journal:  Biochem J       Date:  1997-05-01       Impact factor: 3.857

5.  Preparative high-resolution two-dimensional electrophoresis enables the identification of RNA polymerase B transcription factor 3 as an apoptosis-associated protein in the human BL60-2 Burkitt lymphoma cell line.

Authors:  E Brockstedt; A Otto; A Rickers; K Bommert; B Wittmann-Liebold
Journal:  J Protein Chem       Date:  1999-02

6.  Amino Acid and Peptide Utilization Profiles of the Fluoroacetate-Degrading Bacterium Synergistetes Strain MFA1 Under Varying Conditions.

Authors:  Lex E X Leong; Stuart E Denman; Philip Hugenholtz; Christopher S McSweeney
Journal:  Microb Ecol       Date:  2015-06-26       Impact factor: 4.552

7.  Wood contains a cell-wall structural protein.

Authors:  W Bao; D M O'Malley; R R Sederoff
Journal:  Proc Natl Acad Sci U S A       Date:  1992-07-15       Impact factor: 11.205

8.  Cloning, expression, and characterization of a synthetic analog to the bioadhesive precursor protein of the sea mussel Mytilus edulis.

Authors:  A J Salerno; I Goldberg
Journal:  Appl Microbiol Biotechnol       Date:  1993-05       Impact factor: 4.813

9.  The high concentration of Arg213-->Gly extracellular superoxide dismutase (EC-SOD) in plasma is caused by a reduction of both heparin and collagen affinities.

Authors:  Steen V Petersen; Dorte Aa Olsen; John M Kenney; Tim D Oury; Zuzana Valnickova; Ida B Thøgersen; James D Crapo; Jan J Enghild
Journal:  Biochem J       Date:  2005-01-15       Impact factor: 3.857

10.  Nucleoside diphosphate kinase from Escherichia coli.

Authors:  N Almaula; Q Lu; J Delgado; S Belkin; M Inouye
Journal:  J Bacteriol       Date:  1995-05       Impact factor: 3.490

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