Literature DB >> 3578757

Protein assay sensitive at nanogram levels.

C M Stoscheck.   

Abstract

A simple, fast protein assay which utilizes the affinity of colloidal gold for proteins is described. This assay is sensitive at the 20-ng level when a visible light spectrophotometer is used to measure absorbance. Few chemicals interfere with the assay. Interfering reagents include those that are strongly alkaline, contain high levels of salt, or contain sodium dodecyl sulfate. The problem of alkaline interference can be overcome by acidifying the protein solution before performing the assay. Purified proteins have different capacities to interact with the colloidal gold but this variability is not greater than that seen with the Bradford protein assay.

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Year:  1987        PMID： 3578757     DOI： 10.1016/0003-2697(87)90051-0

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  16 in total

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2.  Membrane bilayer assembly in neural tissue of rat and squid as a critical phenomenon: influence of temperature and membrane proteins.

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Authors:  Jesse Rinehart; Bethany Krett; Mary Anne T Rubio; Juan D Alfonzo; Dieter Söll
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4.  The Mg(2+)-ATPase of rabbit skeletal-muscle transverse tubule is a highly glycosylated multiple-subunit enzyme.

Authors:  T L Kirley
Journal:  Biochem J       Date:  1991-09-01       Impact factor: 3.857

5.  Membrane binding sites for the human blood group H-type 2 determinant, an inducer of laminarinase activity in protoplasts of Rubus fruticosus L.

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6.  Intracellular activation of gelatinase A (72-kDa type IV collagenase) by normal fibroblasts.

Authors:  A Y Lee; K T Akers; M Collier; L Li; A Z Eisen; J L Seltzer
Journal:  Proc Natl Acad Sci U S A       Date:  1997-04-29       Impact factor: 11.205

7.  Heat-stable protein that stimulates acid alpha-glucosidase.

Authors:  N S Radin; A Shukla; G S Shukla; A Sano
Journal:  Biochem J       Date:  1989-12-15       Impact factor: 3.857

8.  Proline residues link the active site to transmembrane domain movements in human nucleoside triphosphate diphosphohydrolase 3 (NTPDase3).

Authors:  Keith J Gaddie; Terence L Kirley
Journal:  Purinergic Signal       Date:  2010-03-30       Impact factor: 3.765

9.  Conserved polar residues stabilize transmembrane domains and promote oligomerization in human nucleoside triphosphate diphosphohydrolase 3.

Authors:  Keith J Gaddie; Terence L Kirley
Journal:  Biochemistry       Date:  2009-10-13       Impact factor: 3.162

10.  A modified protein assay from microgram to low nanogram levels in dilute samples.

Authors:  Ghanshyam D Heda; Upasana Kunwar; Rajiv P Heda
Journal:  Anal Biochem       Date:  2013-10-14       Impact factor: 3.365
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