Literature DB >> 35787

Purification of uricase from mammalian tissue.

T G Conley, D G Priest.   

Abstract

A simple, rapid procedure for the purification of uricase from mammalian tissue is reported. The procedure is based on the precipitation of mammalian uricase under certain dialysis conditions, and on its low solubility near neutral pH. Exceptionally high yields of homogeneous enzyme are obtained.

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Year:  1979        PMID: 35787     DOI: 10.1080/00327487908061683

Source DB:  PubMed          Journal:  Prep Biochem        ISSN: 0032-7484


  4 in total

1.  Rat urate oxidase produced by recombinant baculovirus expression: formation of peroxisome crystalloid core-like structures.

Authors:  K Alvares; R J Widrow; G M Abu-Jawdeh; J V Schmidt; A V Yeldandi; M S Rao; J K Reddy
Journal:  Proc Natl Acad Sci U S A       Date:  1992-06-01       Impact factor: 11.205

2.  Urate oxidase: primary structure and evolutionary implications.

Authors:  X W Wu; C C Lee; D M Muzny; C T Caskey
Journal:  Proc Natl Acad Sci U S A       Date:  1989-12       Impact factor: 11.205

3.  Thermodynamics and stoicheiometry of the binding of substrate analogues to uricase.

Authors:  T G Conley; D G Priest
Journal:  Biochem J       Date:  1980-06-01       Impact factor: 3.857

4.  Statistical media optimization for the production of clinical uricase from Bacillus subtilis strain SP6.

Authors:  Sneha O Pustake; Prashant K Bhagwat; Padma B Dandge
Journal:  Heliyon       Date:  2019-05-21
  4 in total

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