Odunayo Anthonia Taiwo1, Oluwatosin Adebisi Dosumu2, Omoyemi Victoria Akomolafe1, Eniola Oluwayemisi Oni3, Adefemi Oluwasegun Adefuye2, Abiodun Abayomi Shofunde4, Omolola Mary Samuel5, Oluwafemi Adeleke Ojo6. 1. Department of Biochemistry, Chrisland University, Owode, Abeokuta 110222, Nigeria. 2. Department of Biochemistry, Federal University of Agriculture, Abeokuta 110124, Nigeria. 3. Department of Microbiology, Federal University of Agriculture, Abeokuta 110124, Nigeria. 4. Department of Microbiology, Chrisland University, Owode, Abeokuta 110222, Nigeria. 5. Department of Biochemistry, Lead City university, Ibadan 200255, Nigeria. 6. Phytomedicine, Molecular Toxicology, and Computational Biochemistry Research Group, Department of Biochemistry, Bowen University, Iwo, 232101, Nigeria.
Abstract
Background: Automobile repair workshops contribute immensely to the generation of soil and water contamination. This study was conducted to compare the soil microbial load, heavy metals, and consequent toxicological effects, in three (3) automobile mechanic sites. Method: Soil samples were randomly collected from 3 different auto mechanic workshop in Abeokuta town of Ogun-State, Nigeria. Bacterial and fungal counts were done via standard procedures. High-performance liquid chromatography was employed for the aflatoxin quantification. Also, 24 Wistar rats were divided into 4 groups (n = 6), group 1-Control animals: orally administered distilled water, Group 2-administered soil sample solution from Ita Oshin mechanic site (I M), Group 3-administered soil sample solution from Ajebo mechanic site (A M), while Group 4-administered soil sample solution from Laderin mechanic site (L M), for two (2) weeks. Conventional methods were used to determine some physical and biochemical parameters in the rat's serum and tissues. Results: Eight bacterial and fungal genera were identified from the soil samples with Bacillus subtilis and Aspergillus niger occurring most frequently. The levels of heavy metals (lead, zinc, chromium, and cadmium) analyzed were higher than the WHO permissible heavy metal limits in all samples. The activity of liver function enzymes ALP, AST, and ALT was significantly increased in the serum of animals exposed to the 3 soil solution samples when compared with the control group, with the highest recorded at Site II. Conclusion: High level of heavy metals and aflatoxins could predispose to several health-related hazards when humans are exposed to contaminated soil solutions around and within automobile mechanic areas.
Background: Automobile repair workshops contribute immensely to the generation of soil and water contamination. This study was conducted to compare the soil microbial load, heavy metals, and consequent toxicological effects, in three (3) automobile mechanic sites. Method: Soil samples were randomly collected from 3 different auto mechanic workshop in Abeokuta town of Ogun-State, Nigeria. Bacterial and fungal counts were done via standard procedures. High-performance liquid chromatography was employed for the aflatoxin quantification. Also, 24 Wistar rats were divided into 4 groups (n = 6), group 1-Control animals: orally administered distilled water, Group 2-administered soil sample solution from Ita Oshin mechanic site (I M), Group 3-administered soil sample solution from Ajebo mechanic site (A M), while Group 4-administered soil sample solution from Laderin mechanic site (L M), for two (2) weeks. Conventional methods were used to determine some physical and biochemical parameters in the rat's serum and tissues. Results: Eight bacterial and fungal genera were identified from the soil samples with Bacillus subtilis and Aspergillus niger occurring most frequently. The levels of heavy metals (lead, zinc, chromium, and cadmium) analyzed were higher than the WHO permissible heavy metal limits in all samples. The activity of liver function enzymes ALP, AST, and ALT was significantly increased in the serum of animals exposed to the 3 soil solution samples when compared with the control group, with the highest recorded at Site II. Conclusion: High level of heavy metals and aflatoxins could predispose to several health-related hazards when humans are exposed to contaminated soil solutions around and within automobile mechanic areas.
Authors: Gregory J Keir; Benjamin Garfield; David M Hansell; Michael R Loebinger; Robert Wilson; Elisabetta A Renzoni; Athol U Wells; Toby M Maher Journal: Thorax Date: 2013-09-12 Impact factor: 9.139
Authors: Matthew A Sparks; Steven D Crowley; Susan B Gurley; Maria Mirotsou; Thomas M Coffman Journal: Compr Physiol Date: 2014-07 Impact factor: 9.090