Y Zhou1,2,3, Y Ren4,3, G Dai1, X Li1, Y Xiang1, J Zhang1, Y Jiang1, S Jiang1, X Hou1, Z Zhu1, R Wu1. 1. Department of Veterinary Medicine, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing 163319, China. 2. Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, Daqing 163319, China. 3. These authors contributed equally in this study. 4. Dapartment of Pharmacy, College of Daqing, Harbin Medical University, Daqing 163319, China.
Abstract
Background: Heilongjiang province is the main cattle-producing area in China, and molecular epidemiological studies of bovine viral diarrhea viruses (BVDV) in cattle have not been performed in the province. Aims: The objective of this research was to determine the genetic and clinical characteristics of BVDV in cattle. Methods: Fifty-three BVDV-positive clinical samples were collected from 22 cattle farms in Heilongjiang, and the 5´-untranslated region (5´-UTR) was used to carry out a phylogenetic analysis of the viruses. Results: The similarity of the 5´-UTR sequences among these BVDVs was 84.2%-100%, and the phylogenetic analysis showed that all viruses belong to the BVDV-1 species, which is classified into five subtypes: BVDV-1b (47.17%, n=25), 1c (15.09%, n=8), 1d (16.98%, n=9), 1 m (3.77%, n=2), and 1o (16.98%, n=9). The statistical results showed that the BVDV-1b subtype had a positive correlation with gastrointestinal disease (P<0.05; 95% CI: 1.19 to 3.34). There were up to three or four BVDV-1 subtypes in some dairy cattle farms, but farms with a single subtype were prevalent (5/10). Conclusion: BVDV-1b is predominant in cattle herds of Heilongjiang province, China, and shows a positive correlation with gastrointestinal disease. BVDV-1o was found for the first time in Chinese cattle, which increased the complex distribution of BVDV-1 subtypes in cattle herds of China.
Background: Heilongjiang province is the main cattle-producing area in China, and molecular epidemiological studies of bovine viral diarrhea viruses (BVDV) in cattle have not been performed in the province. Aims: The objective of this research was to determine the genetic and clinical characteristics of BVDV in cattle. Methods: Fifty-three BVDV-positive clinical samples were collected from 22 cattle farms in Heilongjiang, and the 5´-untranslated region (5´-UTR) was used to carry out a phylogenetic analysis of the viruses. Results: The similarity of the 5´-UTR sequences among these BVDVs was 84.2%-100%, and the phylogenetic analysis showed that all viruses belong to the BVDV-1 species, which is classified into five subtypes: BVDV-1b (47.17%, n=25), 1c (15.09%, n=8), 1d (16.98%, n=9), 1 m (3.77%, n=2), and 1o (16.98%, n=9). The statistical results showed that the BVDV-1b subtype had a positive correlation with gastrointestinal disease (P<0.05; 95% CI: 1.19 to 3.34). There were up to three or four BVDV-1 subtypes in some dairy cattle farms, but farms with a single subtype were prevalent (5/10). Conclusion: BVDV-1b is predominant in cattle herds of Heilongjiang province, China, and shows a positive correlation with gastrointestinal disease. BVDV-1o was found for the first time in Chinese cattle, which increased the complex distribution of BVDV-1 subtypes in cattle herds of China.
Authors: Robert W Fulton; Julia F Ridpath; Jeremiah T Saliki; Robert E Briggs; Anthony W Confer; Lurinda J Burge; C W Purdy; Raymond W Loan; Glenn C Duff; Mark E Payton Journal: Can J Vet Res Date: 2002-07 Impact factor: 1.310
Authors: Peter Simmonds; Paul Becher; Jens Bukh; Ernest A Gould; Gregor Meyers; Tom Monath; Scott Muerhoff; Alexander Pletnev; Rebecca Rico-Hesse; Donald B Smith; Jack T Stapleton Journal: J Gen Virol Date: 2017-01 Impact factor: 3.891
Authors: Donald B Smith; Gregor Meyers; Jens Bukh; Ernest A Gould; Thomas Monath; A Scott Muerhoff; Alexander Pletnev; Rebecca Rico-Hesse; Jack T Stapleton; Peter Simmonds; Paul Becher Journal: J Gen Virol Date: 2017-08-08 Impact factor: 3.891