Literature DB >> 35781960

Bessel-beam illumination Raman microscopy.

Kazuki Bando1, Shumpei Yabuuchi1, Menglu Li1,2, Toshiki Kubo1, Ryosuke Oketani1, Nicholas I Smith3, Katsumasa Fujita1,2,4.   

Abstract

We demonstrate the use of Bessel beams for side illumination slit-scanning Raman imaging for label-free and hyperspectral analysis of cell spheroids. The background elimination by the side illumination and the aberration-resistant Bessel beam drastically improves the image contrast in Raman observation, allowing label-free investigation of intracellular molecules in thick biological samples. Live cell spheroids were observed to confirm the improvement in image contrast and background reduction with Bessel illumination compared to conventional epi-line illumination.
© 2022 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement.

Entities:  

Year:  2022        PMID： 35781960      PMCID： PMC9208613          DOI： 10.1364/BOE.456138

Source DB:  PubMed          Journal:  Biomed Opt Express        ISSN： 2156-7085            Impact factor:   3.562


  29 in total

1.  Automated method for subtraction of fluorescence from biological Raman spectra.

Authors:  Chad A Lieber; Anita Mahadevan-Jansen
Journal:  Appl Spectrosc       Date:  2003-11       Impact factor: 2.388

2.  Optical sectioning deep inside live embryos by selective plane illumination microscopy.

Authors:  Jan Huisken; Jim Swoger; Filippo Del Bene; Joachim Wittbrodt; Ernst H K Stelzer
Journal:  Science       Date:  2004-08-13       Impact factor: 47.728

3.  Three-dimensional point spread function model for line-scanning confocal microscope with high-aperture objective.

Authors:  E Dusch; T Dorval; N Vincent; M Wachsmuth; A Genovesio
Journal:  J Microsc       Date:  2007-11       Impact factor: 1.758

Review 4.  The third dimension bridges the gap between cell culture and live tissue.

Authors:  Francesco Pampaloni; Emmanuel G Reynaud; Ernst H K Stelzer
Journal:  Nat Rev Mol Cell Biol       Date:  2007-10       Impact factor: 94.444

5.  Label-free biochemical imaging of heart tissue with high-speed spontaneous Raman microscopy.

Authors:  Mitsugu Ogawa; Yoshinori Harada; Yoshihisa Yamaoka; Katsumasa Fujita; Hitoshi Yaku; Tetsuro Takamatsu
Journal:  Biochem Biophys Res Commun       Date:  2009-03-11       Impact factor: 3.575

6.  Gaussian beam in two-photon fluorescence imaging of rat brain microvessel.

Authors:  Lingyan Shi; Adrián Rodríguez-Contreras; Robert R Alfano
Journal:  J Biomed Opt       Date:  2014-12       Impact factor: 3.170

Review 7.  Progress and potential in organoid research.

Authors:  Giuliana Rossi; Andrea Manfrin; Matthias P Lutolf
Journal:  Nat Rev Genet       Date:  2018-11       Impact factor: 53.242

Review 8.  Spheroid culture as a tool for creating 3D complex tissues.

Authors:  Eelco Fennema; Nicolas Rivron; Jeroen Rouwkema; Clemens van Blitterswijk; Jan de Boer
Journal:  Trends Biotechnol       Date:  2013-01-18       Impact factor: 19.536

9.  Label-free biomedical imaging with high sensitivity by stimulated Raman scattering microscopy.

Authors:  Christian W Freudiger; Wei Min; Brian G Saar; Sijia Lu; Gary R Holtom; Chengwei He; Jason C Tsai; Jing X Kang; X Sunney Xie
Journal:  Science       Date:  2008-12-19       Impact factor: 47.728

10.  Spectral tracing of deuterium for imaging glucose metabolism.

Authors:  Luyuan Zhang; Lingyan Shi; Yihui Shen; Yupeng Miao; Mian Wei; Naixin Qian; Yinong Liu; Wei Min
Journal:  Nat Biomed Eng       Date:  2019-04-29       Impact factor: 25.671
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